Document Detail


The effects of refractive index heterogeneity within kidney tissue on multiphoton fluorescence excitation microscopy.
MedLine Citation:
PMID:  21118239     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Although multiphoton fluorescence excitation microscopy has improved the depth at which useful fluorescence images can be collected in biological tissues, the reach of multiphoton fluorescence excitation microscopy is nonetheless limited by tissue scattering and spherical aberration. Scattering can be reduced in fixed samples by mounting in a medium whose refractive index closely matches that of the fixed material. Using optical 'clearing', the effects of refractive index heterogeneity on signal attenuation with depth are investigated. Quantitative measurements show that by mounting kidney tissue in a high refractive index medium, less than 50% of signal attenuates in 100 μm of depth.
Authors:
P A Young; S G Clendenon; J M Byars; K W Dunn
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Publication Detail:
Type:  Journal Article     Date:  2010-09-27
Journal Detail:
Title:  Journal of microscopy     Volume:  242     ISSN:  1365-2818     ISO Abbreviation:  J Microsc     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  England    
Other Details:
Languages:  eng     Pagination:  148-56     Citation Subset:  IM    
Copyright Information:
© 2010 The Authors Journal of Microscopy © 2010 The Royal Microscopical Society.
Affiliation:
Department of Medicine, Division of Nephrology, Indiana University School of Medicine, Indianapolis, Indiana, U.S.A. Department of Physics, Indiana University, Bloomington, Indiana, U.S.A.
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