Document Detail


The effects of a protease inhibitor fraction from tepary bean (Phaseolus acutifolius) on in vitro cell proliferation and cell adhesion of transformed cells.
MedLine Citation:
PMID:  12020595     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Some protease inhibitors (PI), such as the soybean Bowman-Birk protease inhibitor (SBBI), have been described as anticarcinogenic agents. Although PI are ubiquitous compounds in living organisms, the anticarcinogenic potential of PIs other than SBBI remain poorly explored. We evaluated the antiproliferative effect of a protein fraction from tepary bean (Phaseolus acutifolius) seeds with protease inhibitor activity (TPIF), on normal and on malignant cells. TPIF was obtained after precipitation with ammonium sulfate and gel filtration, and its bioactivity was assayed in vitro on HeLa cells, normal 3T3 fibroblasts and 3T3/v-mos transformed fibroblasts. TPIF showed antiproliferative and cytotoxic effects on 3T3/v-mos transformed fibroblasts in a dose-dependent way. On the contrary, TPIF was only cytostatic for normal 3T3 cells at the highest doses assayed, and had no effect on epithelial HeLa cells proliferation. Sublethal TPIF doses also stimulated cell adhesion of poorly adherent 3T3/v-mos cell line.
Authors:
T García-Gasca; L A Salazar-Olivo; E Mendiola-Olaya; A Blanco-Labra
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  16     ISSN:  0887-2333     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-05-21     Completed Date:  2002-11-14     Revised Date:  2009-04-10    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  229-33     Citation Subset:  IM    
Affiliation:
School of Chemistry, Universidad Autónoma de Querétaro, Querétaro 76010, Qro., Mexico.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Cell Adhesion / drug effects
Cell Division / drug effects
Cell Line, Transformed / drug effects*,  pathology
Cytotoxins / toxicity*
Dose-Response Relationship, Drug
Electrophoresis, Polyacrylamide Gel
Fibroblasts / drug effects,  pathology
Hela Cells
Humans
Mice
Phaseolus*
Plant Extracts / chemistry
Protease Inhibitors / toxicity*
Chemical
Reg. No./Substance:
0/Cytotoxins; 0/Plant Extracts; 0/Protease Inhibitors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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