| The effects of a protease inhibitor fraction from tepary bean (Phaseolus acutifolius) on in vitro cell proliferation and cell adhesion of transformed cells. | |
| | |
MedLine Citation:
|
PMID: 12020595 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Some protease inhibitors (PI), such as the soybean Bowman-Birk protease inhibitor (SBBI), have been described as anticarcinogenic agents. Although PI are ubiquitous compounds in living organisms, the anticarcinogenic potential of PIs other than SBBI remain poorly explored. We evaluated the antiproliferative effect of a protein fraction from tepary bean (Phaseolus acutifolius) seeds with protease inhibitor activity (TPIF), on normal and on malignant cells. TPIF was obtained after precipitation with ammonium sulfate and gel filtration, and its bioactivity was assayed in vitro on HeLa cells, normal 3T3 fibroblasts and 3T3/v-mos transformed fibroblasts. TPIF showed antiproliferative and cytotoxic effects on 3T3/v-mos transformed fibroblasts in a dose-dependent way. On the contrary, TPIF was only cytostatic for normal 3T3 cells at the highest doses assayed, and had no effect on epithelial HeLa cells proliferation. Sublethal TPIF doses also stimulated cell adhesion of poorly adherent 3T3/v-mos cell line. |
| | |
Authors:
|
T García-Gasca; L A Salazar-Olivo; E Mendiola-Olaya; A Blanco-Labra |
Related Documents
:
|
197085 - Calcium stimulation of plasminogen activator secretion/production by swiss 3t3 cells. 9468115 - Intracellular melatonin distribution in cultured cell lines. 20512465 - The inhibitory effect of ginseng pectin on l-929 cell migration. 145445 - Variation in potassium transport properties of mouse 3t3 cells as a result of subcultiv... 20393015 - Establishment and characterization of multidrug-resistant gastric cancer cell lines. 6618005 - Cpk accumulation in fusion-blocked quail myocytes. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 16 ISSN: 0887-2333 ISO Abbreviation: Toxicol In Vitro Publication Date: 2002 Jun |
Date Detail:
|
Created Date: 2002-05-21 Completed Date: 2002-11-14 Revised Date: 2009-04-10 |
Medline Journal Info:
|
Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
|
Languages: eng Pagination: 229-33 Citation Subset: IM |
Affiliation:
|
School of Chemistry, Universidad Autónoma de Querétaro, Querétaro 76010, Qro., Mexico. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
3T3 Cells Animals Cell Adhesion / drug effects Cell Division / drug effects Cell Line, Transformed / drug effects*, pathology Cytotoxins / toxicity* Dose-Response Relationship, Drug Electrophoresis, Polyacrylamide Gel Fibroblasts / drug effects, pathology Hela Cells Humans Mice Phaseolus* Plant Extracts / chemistry Protease Inhibitors / toxicity* |
| Chemical | |
Reg. No./Substance:
|
0/Cytotoxins; 0/Plant Extracts; 0/Protease Inhibitors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Comparative effect of colchicine and colchiceine on cytotoxicity and CYP gene expression in primary ...
Next Document: In vitro toxicity of 7H-dibenzo[c,g]carbazole in human liver cell lines.