| The effects of pressure on yeast cytochrome c peroxidase. | |
| | |
MedLine Citation:
|
PMID: 3017716 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The effects of pressure on cytochrome c peroxidase [CcP(FeIII)], its cyano derivative (CcP X CN) and its enzyme-substrate complex (ES) have been studied. The effects of pressure on the binding of the substrate analog porphyrin cytochrome c (porphyrin c) to CcP X CN and ES have also been studied. High pressure causes CcP(FeIII) to undergo a high-spin to low-spin transition but has no detectable effect on either CcP X CN, which is already low spin, or on ES. The low-spin CcP(FeIII) structure at pressure is similar to the low-spin form at low temperature and the low-spin form of horseradish peroxidase at high pressure. delta V degree associated with the spin equilibrium is about 30 ml/mol and is independent of temperature. delta G degree is small, 4.7 kJ/mol at 0 degree C, while delta H degree is 14.2 kJ/mol at 1 bar (100 kPa). Pressure has no detectable effect on the binding equilibria of mixtures of CcP X CN plus porphyrin c or ES plus porphyrin c. This indicates that the interaction of CcP and porphyrin c results in little or no volume change; the same is true in the case of cytochrome c oxidase and porphyrin c. |
| | |
Authors:
|
J A Kornblatt; A M English; G Hui Bon Hoa |
Related Documents
:
|
16487756 - High-pressure macromolecular crystallography (hpmx): status and prospects. 17196696 - Inactivation kinetics of selected aerobic and anaerobic bacterial spores by pressure-as... 17455336 - Demonstration of a shear-based solid-state phase transformation in a small molecular or... 16846506 - Observation of intermediate states of the human prion protein by high pressure nmr spec... 7690386 - Gutron treatment of patients suffering from essential orthostatic hypotony. part three-... 19875426 - Effect of tailored practice and patient care plans on secondary prevention of heart dis... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: European journal of biochemistry / FEBS Volume: 159 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 1986 Aug |
Date Detail:
|
Created Date: 1986-10-23 Completed Date: 1986-10-23 Revised Date: 2007-07-23 |
Medline Journal Info:
|
Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: GERMANY, WEST |
Other Details:
|
Languages: eng Pagination: 39-43 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cytochrome-c Peroxidase
/
metabolism* Peroxidases / metabolism* Porphyrins / metabolism Pressure Protein Binding Saccharomyces cerevisiae / enzymology* Spectrophotometry Thermodynamics |
| Chemical | |
Reg. No./Substance:
|
0/Porphyrins; 635-50-7/porphyrin c; EC 1.11.1.-/Peroxidases; EC 1.11.1.5/Cytochrome-c Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Tarantula hemocyanin mRNA. In vitro translation, cDNA cloning and nucleotide sequence corresponding ...
Next Document: Interaction of vasoactive intestinal peptide (VIP) and N-terminally modified VIP analogs with rat pa...