| The effects of prenatal administration of azo dyes on testicular development in the mouse: a structure activity profile of dyes derived from benzidine, dimethylbenzidine, or dimethoxybenzidine. | |
MedLine Citation:
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PMID: 8449389 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In mice and rats, prenatal exposure to the dye Congo red permanently reduces the number of germ cells in male and female offspring. In the current investigation, nine other dyes structurally related to Congo red were examined for developmental testicular toxicity. In this study, the structural component of the dyes responsible for the prenatal induction of germ cell aplasia was identified. We found that only benzidine-based dyes altered testicular development and caused hypospermatogenesis in mice during adulthood. Dimethyl- and dimethoxybenzidine-based dyes were without effect. Pregnant mice were dosed orally on Days 8-12 of gestation with a benzidine-, dimethylbenzidine-, or a dimethoxybenzidine-based dye and the testes of 45- to 50-day-old male offspring were examined. The testes of postpubertal male offspring exposed to the benzidine-based dyes, Congo red, diamine blue, and Chlorazol Black E, were small and contained some tubules completely devoid of germ cells, but the dimethylbenzidine-based dyes, trypan blue, Evans blue, and benzopurpurin 4B, and the dimethoxybenzidine-based dye, Chicago sky blue, did not alter testicular development in this manner. Azoic diazo component 48, a dimethoxybenzidine congener, and two other diazo dyes, naphthol blue black and Sudan III, were also without effect on the germ cells. Experiments with Chlorazol Black E (CBE) indicate that the period of susceptibility in the male fetus is limited to the period of primordial germ cell migration and division. When CBE was administered on Days 8-10 of gestation it reduced testis weight after puberty by 30%, while treatment after Day 13 did not affect testicular function.(ABSTRACT TRUNCATED AT 250 WORDS) |
Authors:
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L E Gray; J S Ostby |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Fundamental and applied toxicology : official journal of the Society of Toxicology Volume: 20 ISSN: 0272-0590 ISO Abbreviation: Fundam Appl Toxicol Publication Date: 1993 Feb |
Date Detail:
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Created Date: 1993-04-15 Completed Date: 1993-04-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8200838 Medline TA: Fundam Appl Toxicol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 177-83 Citation Subset: IM |
Affiliation:
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Developmental Reproductive Biology Section, RTB, DTD, USEPA, Research Triangle Park, North Carolina 27711. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Abnormalities, Drug-Induced
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etiology Aging / physiology Animals Azo Compounds / toxicity* Benzidines / toxicity* Body Weight / drug effects Coloring Agents / toxicity* Dianisidine / toxicity* Epididymis / drug effects Female Male Mice Organ Size / drug effects Pregnancy Prenatal Exposure Delayed Effects* Spermatogenesis / drug effects Structure-Activity Relationship Testis / anatomy & histology, drug effects*, growth & development |
| Chemical | |
Reg. No./Substance:
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0/Azo Compounds; 0/Benzidines; 0/Coloring Agents; 119-90-4/Dianisidine; 119-93-7/2-tolidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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