Document Detail

The effects of potassium, 5-hydrocytryptamine, adrenocorticotrophin and angiotensin II on the concentration of adenosine 3':5'-cyclic monophosphate in suspensions of dispersed rat adrenal zona glomerulosa and zona fasciculata cells.
MedLine Citation:
PMID:  4374199     Owner:  NLM     Status:  MEDLINE    
Dispersed rat adrenal cells prepared from both the capsule and the decapsulated gland were used to investigate the effects on cyclic AMP accumulation of known stimuli of steroidogenesis [ACTH (adrenocorticotrophin), angiotensin II, K(+) ions and 5-hydroxytryptamine]. Since glomerulosa-cell preparations from capsular strippings are normally contaminated with a proportion of fasciculata cells, cells purified by fractionation on a bovine serum albumin gradient were also used. The results showed that: (1) ACTH and angiotensin II stimulated cyclic AMP accumulation in both fractionated and unfractionated zona fasciculata cells; (2) 5-hydroxytryptamine and an increased extracellular K(+) concentration (from 3.6 to 8.4mm) had no effect on cyclic AMP concentrations in fasciculata cell preparations; (3) the addition of ACTH, angiotensin II, 5-hydroxytryptamine or K(+) to the incubation medium resulted in increased cyclic AMP concentrations in unpurified zona glomerulosa cell preparations; (4) fractionation and hence the virtual elimination of fasciculata contamination, did not affect the response to 5-hydroxytryptamine and increased K(+) concentration. However, the responses to ACTH and angiotensin II were markedly lowered but not abolished. These results strongly suggest a link between cyclic AMP production and steroidogenesis in the zone of the adrenal gland that specifically secretes aldosterone. All four agents used stimulated both steroid output and cyclic AMP accumulation. However, at certain doses of 5-hydroxytryptamine, K(+) and angiotensin II the significant increases in corticosterone output were not accompanied by measurable increases in cyclic AMP accumulation.
J D Albano; B L Brown; R P Ekins; S A Tait; J F Tait
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  142     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1974 Aug 
Date Detail:
Created Date:  1975-03-18     Completed Date:  1975-03-18     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  391-400     Citation Subset:  IM    
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MeSH Terms
Adrenal Glands / drug effects*,  metabolism,  secretion
Adrenocorticotropic Hormone / pharmacology*
Aldosterone / secretion
Angiotensin II / pharmacology*
Chromatography, Ion Exchange
Cyclic AMP / metabolism*
Microscopy, Electron
Potassium / pharmacology*
Serotonin / pharmacology*
Serum Albumin, Bovine
Steroids / biosynthesis
Reg. No./Substance:
0/Serum Albumin, Bovine; 0/Steroids; 10028-17-8/Tritium; 11128-99-7/Angiotensin II; 50-67-9/Serotonin; 52-39-1/Aldosterone; 60-92-4/Cyclic AMP; 7440-09-7/Potassium; 9002-60-2/Adrenocorticotropic Hormone

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