Document Detail


The effects of pig follicular fluid upon the ultrastructure of rat granulosa cells in tissue culture.
MedLine Citation:
PMID:  7349459     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ultrastructure of rat granulosa cells from preovulatory follicles has been investigated after incubation in control fetal calf serum (FCS) medium with or without LH (1 micron/ml), and in total or steroid-free pig follicular fluid (FF) medium. While the cells had a similar ultrastructure after 1 day of incubation in these media, significant differences were detectable after 3 days. In FCS supplemented with LH, autolysis and phagocytotic activity remained elevated, and cells did not develop any of the typical ultrastructural features of steroid-secreting cells. In contrast, total FF induced complete differentiation of mitochondrial cristae from the lamelliform to the villiform type. Steroid-free FF induced the same array of effects as total FF, except that mitochondrial differentiation was not complete. These observations together with those of previous studies, in which progesterone secretion was measured in cells incubated in the same media, suggested that the development of the capacity to secrete progesterone in rat luteal cells could be directly related only to mitochondrial differentiation.
Authors:
J Bernard; H Merchant-Larios
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction, nutrition, development     Volume:  20     ISSN:  0181-1916     ISO Abbreviation:  Reprod Nutr Dev     Publication Date:  1980  
Date Detail:
Created Date:  1983-03-11     Completed Date:  1983-03-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8005903     Medline TA:  Reprod Nutr Dev     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  905-21     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Fluids / physiology*
Cells, Cultured
Culture Media
Cytoplasm / ultrastructure
Female
Granulosa Cells / drug effects,  ultrastructure*
Luteinizing Hormone / pharmacology
Microscopy, Electron
Microtubules / ultrastructure
Mitochondria / ultrastructure
Ovarian Follicle / physiology*
Phagocytosis
Rats
Rats, Inbred Strains
Steroids / physiology
Swine
Chemical
Reg. No./Substance:
0/Culture Media; 0/Steroids; 9002-67-9/Luteinizing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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