Document Detail


The effects of metals and food availability on the behavior of Caenorhabditis elegans.
MedLine Citation:
PMID:  14713049     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Caenorhabditis elegans, a nonparasitic soil nematode, was used to assess the combined effects of metal exposures and food availability on behavior. Movement was monitored using a computer tracking system after exposures to Cu, Pb, or Cd while feeding was measured as a change in optical density (deltaOD) of bacteria suspensions over the exposure period. After 24-h exposures at high and low bacteria concentrations, movement was decreased in a concentration-dependent fashion by Pb and Cd but feeding reductions were not directly proportional to exposure concentrations. Copper exposure induced concentration-dependent declines in feeding and movement regardless of bacteria concentration. The impact of 24-h metal exposures was apparently reduced by increasing food availability. Therefore, exposures were shortened to 4 h in an attempt to minimize starvation effects on movement. Although nematodes were immobilized following 24 h of food depravation, worms deprived of food during the 4-h exposure continued to feed and move after exposure. A bead-ingestion assay after 4-h exposures was also used as an additional means of assessing the effects of metals on feeding behavior. Ingestion was significantly reduced by all concentrations of metals tested, indicating its sensitivity as a sublethal assay. Feeding (deltaOD) during exposures exhibited similar trends as ingestion but was slightly less sensitive, while movement was the least sensitive assay of 4-h metal exposures to C. elegans. Assessment of multiple sublethal endpoints allowed for the determination of the separate and interactive effects of metals and food availability on C. elegans behavior.
Authors:
Windy A Boyd; Russell D Cole; Gary L Anderson; Phillip L Williams
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Environmental toxicology and chemistry / SETAC     Volume:  22     ISSN:  0730-7268     ISO Abbreviation:  Environ. Toxicol. Chem.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2004-01-09     Completed Date:  2004-04-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8308958     Medline TA:  Environ Toxicol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3049-55     Citation Subset:  IM    
Affiliation:
Department of Environmental Health Science, University of Georgia, Athens, Georgia 30602-2102, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Assay
Cadmium / toxicity*
Caenorhabditis elegans*
Copper / toxicity*
Environmental Exposure*
Feeding Behavior*
Lead / toxicity*
Sensitivity and Specificity
Starvation
Water Pollutants / toxicity*
Chemical
Reg. No./Substance:
0/Water Pollutants; 7439-92-1/Lead; 7440-43-9/Cadmium; 7440-50-8/Copper

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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