Document Detail

The effects of local anaesthetics on retinal function.
MedLine Citation:
PMID:  6983179     Owner:  NLM     Status:  MEDLINE    
Isolated frog eyecups were incubated in Ringer containing local anaesthetics to study the effects of these drugs on dark-adaptation of the ERG. Relative to controls, dark-adaptation in eyecups treated with millimolar concentrations of MS-222, benzocaine, and procaine HCl was significantly inhibited during 10 to 120 min following the cessation of the adapting light. These drugs also prevented the recovery of the c-wave during dark-adaptation, resulting in ERG waveforms resembling those found in light-adapted eyecups. Measurements of rhodopsin in the retina were consistent with previous findings showing that rhodopsin regeneration in situ is inhibited by local anaesthetics. In vitro regeneration experiments in which bleached rod outer segment fragments were added to 11-cis retinal showed that preincubation of retinal with MS-222 in ethanol prevents rhodopsin regeneration. Evidence was obtained spectrophotometrically for the formation of a complex between MS-222 and 11-cis retinal with a gamma max of 512 nm. We propose that the formation of a Schiff's base between these two compounds blocks the recombination of rhodopsin, and in situ, leads to the inhibition of dark-adaptation.
L M Rapp; S F Basinger
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Vision research     Volume:  22     ISSN:  0042-6989     ISO Abbreviation:  Vision Res.     Publication Date:  1982  
Date Detail:
Created Date:  1983-01-19     Completed Date:  1983-01-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0417402     Medline TA:  Vision Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1097-103     Citation Subset:  IM    
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MeSH Terms
Aminobenzoic Acids / pharmacology
Anesthetics, Local / pharmacology*
Benzocaine / pharmacology
Dark Adaptation / drug effects*
Procaine / pharmacology
Rana pipiens
Retina / drug effects,  physiology*
Retinaldehyde / metabolism
Rhodopsin / metabolism
Urethane / pharmacology
Grant Support
EY-01406-06/EY/NEI NIH HHS; EY-05493-01/EY/NEI NIH HHS
Reg. No./Substance:
0/Aminobenzoic Acids; 0/Anesthetics, Local; 116-31-4/Retinaldehyde; 51-79-6/Urethane; 582-33-2/tricaine; 59-46-1/Procaine; 9009-81-8/Rhodopsin; 94-09-7/Benzocaine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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