| The effects of laminin on the growth and differentiation of embryonal carcinoma cells in defined media. | |
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MedLine Citation:
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PMID: 7242089 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this paper we have examined the growth and differentiation of the embryonal carcinoma cell line, F9, in the defined medium EM-3 at low density. We show that the growth of F9 and their differentiated cells (F9-diff) in EM-3 is strongly density dependent. At low cell densities the growth of both cell types is severely limited and most of the cells do not survive. Although this poses a problem for working with F9 and F9-diff in EM-3, it provides a convenient assay for identifying molecules that support their growth at low density. Using this assay, we have determined that laminin, a newly isolated glycoprotein of basement membranes, significantly improves the growth and short-term survival of both F9 and F9-diff. However, addition of laminin to EM-3 is insufficient to promote the clonal growth of these cell types. Our findings also indicate that laminin promotes the attachment of F9 and F9-diff in defined media. On the basis of our results, we propose an attachment function for laminin during the early stages of mammalian development. |
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Authors:
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A Rizzino; V Terranova; D Rohrbach; C Crowley; H Rizzino |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of supramolecular structure Volume: 13 ISSN: 0091-7419 ISO Abbreviation: J. Supramol. Struct. Publication Date: 1980 |
Date Detail:
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Created Date: 1981-08-10 Completed Date: 1981-08-10 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0330464 Medline TA: J Supramol Struct Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 243-53 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Basement Membrane / metabolism Cell Adhesion / drug effects Cell Differentiation / drug effects* Cell Division / drug effects* Cells, Cultured Glycoproteins / pharmacology* Laminin Mice Neoplasms, Experimental / pathology Teratoma / pathology* |
| Grant Support | |
ID/Acronym/Agency:
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5P01 CA 23052/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Glycoproteins; 0/Laminin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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