Document Detail

The effects of laminin on the growth and differentiation of embryonal carcinoma cells in defined media.
MedLine Citation:
PMID:  7242089     Owner:  NLM     Status:  MEDLINE    
In this paper we have examined the growth and differentiation of the embryonal carcinoma cell line, F9, in the defined medium EM-3 at low density. We show that the growth of F9 and their differentiated cells (F9-diff) in EM-3 is strongly density dependent. At low cell densities the growth of both cell types is severely limited and most of the cells do not survive. Although this poses a problem for working with F9 and F9-diff in EM-3, it provides a convenient assay for identifying molecules that support their growth at low density. Using this assay, we have determined that laminin, a newly isolated glycoprotein of basement membranes, significantly improves the growth and short-term survival of both F9 and F9-diff. However, addition of laminin to EM-3 is insufficient to promote the clonal growth of these cell types. Our findings also indicate that laminin promotes the attachment of F9 and F9-diff in defined media. On the basis of our results, we propose an attachment function for laminin during the early stages of mammalian development.
A Rizzino; V Terranova; D Rohrbach; C Crowley; H Rizzino
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of supramolecular structure     Volume:  13     ISSN:  0091-7419     ISO Abbreviation:  J. Supramol. Struct.     Publication Date:  1980  
Date Detail:
Created Date:  1981-08-10     Completed Date:  1981-08-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0330464     Medline TA:  J Supramol Struct     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  243-53     Citation Subset:  IM    
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MeSH Terms
Basement Membrane / metabolism
Cell Adhesion / drug effects
Cell Differentiation / drug effects*
Cell Division / drug effects*
Cells, Cultured
Glycoproteins / pharmacology*
Neoplasms, Experimental / pathology
Teratoma / pathology*
Grant Support
5P01 CA 23052/CA/NCI NIH HHS
Reg. No./Substance:
0/Glycoproteins; 0/Laminin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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