Document Detail


The effects of ivermectin on the viability of Onchocerca lienalis microfilariae in vitro and on their subsequent development in the blackfly vector, Simulium ornatum.
MedLine Citation:
PMID:  2052853     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Experiments examined the sensitivity of Onchocerca lienalis microfilariae (mf) to ivermectin in vitro using a range of parameters to measure viability. Incubation in drug (1 x 10(-4) and 5.71 x 10(-8) M) caused an immediate decrease in motility levels in a concentration dependent fashion with a continuing decline over 24 h; the inclusion of a monkey kidney cell (LLCMK2) feeder layer in the culture system partially abrogated drug effects. The use of feeder cells allowed the longer-term culture of mf; incubation in drug at low concentrations (5.71 x 10(-8) and 1 x 10(-10) M) significantly reduced motility levels during a 17 day trial. Using MTT colorimetry it was found that 10,000 untreated mf per replicate sample were required to produce a sufficient quantity of formazan to make comparisons, and that formazan formation was most rapid during the first 30 min of incubation in MTT but continued up to 3-4 h. Following incubation of mf in ivermectin (1 x 10(-4) and 5.71 x 10(-8) M) for 24 h, only the higher concentration inhibited formazan formation (51%), while motility levels were reduced by 94% and 45% respectively. Using the same drug concentrations, mf viability was assessed by measuring the uptake of [3H]2-deoxy-D-glucose; uptake was only significantly reduced (40%) by the higher concentration, while motility levels were reduced by 77% and 63% respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
S Townson; S K Tagboto
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft für Technische Zusammenarbeit (GTZ)     Volume:  42     ISSN:  0177-2392     ISO Abbreviation:  Trop. Med. Parasitol.     Publication Date:  1991 Mar 
Date Detail:
Created Date:  1991-07-23     Completed Date:  1991-07-23     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8503728     Medline TA:  Trop Med Parasitol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  31-7     Citation Subset:  IM    
Affiliation:
CAB International Institute of Parasitology, St. Albans, Hertfordshire, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Glucose / metabolism
Host-Parasite Interactions / drug effects
Insect Vectors / parasitology*
Ivermectin / pharmacology*
Microfilaria / drug effects,  growth & development,  metabolism
Movement / drug effects
Onchocerca / drug effects*,  growth & development,  physiology
Oxidation-Reduction
Simuliidae / parasitology*
Tetrazolium Salts / metabolism
Chemical
Reg. No./Substance:
0/Tetrazolium Salts; 50-99-7/Glucose; 70288-86-7/Ivermectin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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