Document Detail


The effects of irradiation and temperature on the immunological activity of staphylococcal enterotoxin A.
MedLine Citation:
PMID:  2223523     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of irradiation and temperature on purified staphylococcal enterotoxin A were investigated using sensitive ELISA systems. Thermal inactivation of staphylococcal enterotoxin A in phosphate-buffered saline was considerably faster at temperatures of 60, 70, 115 and 121 degrees C than at 90 and 100 degrees C. In gelatin phosphate buffer, staphylococcal enterotoxin A was completely inactivated by irradiation at 8.0 kGy; in a 15% mince slurry, however, 27-37% of staphylococcal enterotoxin A remained at this level of irradiation. Even at a dose of 23.7 kGy, 16-26% residual staphylococcal enterotoxin A could still be detected. Generally, increasing the mince concentration increased the protection against the effect of irradiation on staphylococcal enterotoxin A. However, the protective effect of mince at a concentration of 50% was less than at a mince concentration of 30%. Both irradiation and heat processing of food should only be used in conjunction with good manufacturing practices to prevent proliferation of microorganisms and toxin productions.
Authors:
N K Modi; S A Rose; H S Tranter
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of food microbiology     Volume:  11     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  1990 Aug 
Date Detail:
Created Date:  1990-12-27     Completed Date:  1990-12-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  85-92     Citation Subset:  IM    
Affiliation:
PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wilts, U.K.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Enterotoxins / analysis,  immunology,  radiation effects*
Enzyme-Linked Immunosorbent Assay
Food Irradiation*
Food Microbiology*
Hot Temperature
Meat
Reagent Kits, Diagnostic
Staphylococcus aureus*
Chemical
Reg. No./Substance:
0/Enterotoxins; 0/Reagent Kits, Diagnostic; 37337-57-8/enterotoxin A, Staphylococcal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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