Document Detail

The effects of ionophores on steroidogenesis and morphology of avian granulosa cells.
MedLine Citation:
PMID:  3607849     Owner:  NLM     Status:  MEDLINE    
Granulosa cells, isolated by collagenase digestion from the mature ovarian follicle of laying hens, were incubated in the presence of two ionophores, lasalocid (X537A) and ionomycin, to determine their effects on basal and stimulated steroidogenesis, as well as their effects on various cell parameters including DNA, RNA, and protein synthesis. Both ionophores caused a dose-dependent inhibition of agonist-promoted progesterone production and, in the presence of calcium, a small but significant increase in basal output of progesterone. Whereas the conversion of pregnenolone to progesterone was unaffected by the ionophores, the activity of cholesterol side-chain cleavage enzyme was inhibited in a dose-related manner. Both ionophores decreased cellular levels of ATP and inhibited the incorporation of radioactively-labeled precursors into DNA, RNA, and proteins. Morphologically, ionophore-treated cells showed swelling of the rough endoplasmic reticulum. Similar morphological changes were also observed in cells treated with oligomycin, a known metabolic inhibitor. These results suggest that the ionophores lasalocid and ionomycin impair release of energy and thereby exert the principal cause of the inhibited steroidogenic response by granulosa cells to a variety of agonists.
R L Zobell; F Hertelendy; V W Fischer
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Cell and tissue research     Volume:  248     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  1987 Jun 
Date Detail:
Created Date:  1987-08-28     Completed Date:  1987-08-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  551-8     Citation Subset:  IM    
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MeSH Terms
Ethers / pharmacology
Granulosa Cells / drug effects,  metabolism*,  ultrastructure
Hydroxycholesterols / metabolism
Lasalocid / pharmacology*
Microscopy, Electron
Mitochondria / metabolism
Progesterone / biosynthesis*
Reg. No./Substance:
0/Ethers; 0/Hydroxycholesterols; 2140-46-7/25-hydroxycholesterol; 25999-31-9/Lasalocid; 56092-81-0/Ionomycin; 57-83-0/Progesterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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