Document Detail

The effects of intrathecal cyclooxygenase-1, cyclooxygenase-2, or nonselective inhibitors on pain behavior and spinal Fos-like immunoreactivity.
MedLine Citation:
PMID:  18292448     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Prostaglandins are synthesized by cyclooxygenase (COX) and are thought to play an important role in nociceptive transmission in the spinal cord. Fos expression is an indicator of spinal neuron activation. We examined the role of intrathecal selective and nonspecific COX inhibitors on spinal C-Fos expression. METHODS: To evaluate the relative contribution of COX-1 and COX-2 in nociceptive transmission in the spinal cord, we assessed the effects of the selective COX-1 inhibitor SC 560, the selective COX-2 inhibitor celecoxib, and the nonselective COX inhibitor ketorolac on formalin-evoked behavior and spinal c-Fos-like immunoreactivity (FLI). Rats received each of the drugs (30, 60, or 90 microg) intrathecally before the subcutaneous administration of formalin (5%, 50 microL) to the plantar surface of a hindpaw. The control group received vehicle intrathecally before the administration of formalin. RESULTS: Phase 1 flinching behavior decreased in rats given celecoxib or ketorolac 90 mug. Phase 2 flinching behavior decreased in rats given all doses of ketorolac or celecoxib 90 microg (P < 0.05). The FLI was significantly reduced in rats given celecoxib or ketorolac 90 microg for laminae I-II (P < 0.05). By contrast, for laminae V-VI, only the ketorolac 60 or 90 microg treatment group demonstrated a larger decrease in FLI (P < 0.05). The FLI expression in laminae V-VI had a significant correlation with phase 2 flinching behavior (P < 0.05). CONCLUSIONS: A dual inhibitor of COX-1 and COX-2 suppressed both responses of formalin-evoked behaviors and FLI expression of whole laminae in the lumbar spinal cord. FLI expression of laminae I-II alone may not be a good indicator of the ability to produce anti-hypersensitivity; however, the FLI of laminae V-VI correlates with phase 2 responses.
Il Ok Lee; Youngsun Seo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anesthesia and analgesia     Volume:  106     ISSN:  1526-7598     ISO Abbreviation:  Anesth. Analg.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-22     Completed Date:  2008-03-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1310650     Medline TA:  Anesth Analg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  972-7, table of contents     Citation Subset:  AIM; IM    
Department of Anesthesiology, 97 Guro-dong gil, Guro-gu, Korea University Guro Hospital, College of Medicine, Korea University, Seoul, Korea 152-703.
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MeSH Terms
Behavior, Animal / drug effects*
Cyclooxygenase 1 / metabolism*
Cyclooxygenase 2 / metabolism*
Cyclooxygenase 2 Inhibitors / administration & dosage*
Cyclooxygenase Inhibitors / administration & dosage*
Dose-Response Relationship, Drug
Hyperalgesia / chemically induced,  prevention & control*
Injections, Spinal
Ketorolac / administration & dosage
Membrane Proteins / metabolism*
Pain / chemically induced,  prevention & control*
Pain Measurement
Posterior Horn Cells / drug effects*,  enzymology,  metabolism
Proto-Oncogene Proteins c-fos / metabolism*
Pyrazoles / administration & dosage
Rats, Sprague-Dawley
Sulfonamides / administration & dosage
Time Factors
Reg. No./Substance:
0/Cyclooxygenase 2 Inhibitors; 0/Cyclooxygenase Inhibitors; 0/Membrane Proteins; 0/Proto-Oncogene Proteins c-fos; 0/Pyrazoles; 0/SC 560; 0/Sulfonamides; 169590-42-5/celecoxib; 50-00-0/Formaldehyde; 66635-83-4/Ketorolac; EC 1; EC 2; EC protein, rat; EC protein, rat

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