Document Detail

The effects of insulin on myocardial metabolism and acidosis in normoxia and ischaemia. A 31P-NMR study.
MedLine Citation:
PMID:  7037057     Owner:  NLM     Status:  MEDLINE    
1. The effect of insulin on the perfused rat heart during normoxia and total ischaemia was studied by 31P-NMR. 2. During normoxic perfusion, insulin increased the phosphocreatine to ATP ratio at the expense of Pi, when glucose was the substrate. No change was observed when acetate was used as the sole substrate. the intracellular pH (as measured from the position of the 2-deoxyglucose 6-phosphate resonance peak) was unaffected by insulin treatment. 3. Infusion of insulin prior to ischaemia caused an increase in the rate and extent of acidosis during the period of no flow while the rate of ATP depletion was decreased. 4. Freeze-clamped studies showed an increase in glycogen levels upon insulin treatment of the glucose perfused rat heart. During ischaemia, a decrease in glycogen content concomitant with an increase in lactate was observed. The accessibility of glycogen to phosphorylase during ischaemia is increased as a result of insulin treatment. The control of glycolysis during ischaemia is discussed with respect to the content and structure of glycogen in heart tissue.
I A Bailey; G K Radda; A M Seymour; S R Williams
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  720     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1982 Feb 
Date Detail:
Created Date:  1982-05-21     Completed Date:  1982-05-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  17-27     Citation Subset:  IM    
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MeSH Terms
Acidosis / metabolism*
Adenosine Triphosphate / metabolism
Coronary Disease / metabolism*
Disease Models, Animal
Glucose / metabolism
Heart / drug effects
Hydrogen-Ion Concentration
Insulin / pharmacology*
Magnetic Resonance Spectroscopy
Myocardium / metabolism*
Phosphates / metabolism
Rats, Inbred Strains
Grant Support
HL 18708--0251/HL/NHLBI NIH HHS
Reg. No./Substance:
0/Phosphates; 11061-68-0/Insulin; 50-99-7/Glucose; 56-65-5/Adenosine Triphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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