Document Detail


The effects of insulin on the level and activity of the GLUT4 present in human adipose cells.
MedLine Citation:
PMID:  7672486     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human adipose cells are much less responsive to insulin stimulation of glucose transport activity than are rat adipocytes. To assess and characterize this difference, we have determined the rates of 3-O-methyl-D-glucose transport in human adipose cells and have compared these with the levels of glucose transporter 4 (GLUT4) assessed by using the bis-mannose photolabel, 2-N-4-(1-azi-2,2,2-trifluoroethyl)benzoyl-1,3-bis-(D-mannos- 4-yloxy)-2-propyl-amine, ATB-BMPA. The rates of 3-O-methyl-D-glucose transport and the cell-surface level of GLUT4 are very similar in the human and rat adipocyte in the basal state. The Vmax for 3-O-methyl-D-glucose transport in fully insulin-stimulated human adipose cells is 15-fold lower than in rat adipose cells. Photolabelling of GLUT4 suggests that this low transport activity is associated with a low GLUT4 abundance (39 x 10(4) sites/cell; 19.9 x 10(4) sites at the cell surface). The turnover number for human adipose cell GLUT4 (5.8 x 10(4) min-1) is similar to that observed for GLUT4 in rat adipose cells and the mouse cell line, 3T3L1. Since 50% of the GLUT4 is at the cell surface of both human and rat adipose cells in the fully insulin-stimulated state, an inefficient GLUT4 exocytosis process cannot account for the low transport activity. The intracellular retention process appears to have adapted to release, in the basal state, a greater proportion of the total-cellular pool of GLUT4 to the cell surface of the larger human adipocytes. These cell-surface transporters are presumably necessary to provide the basal metabolic needs of the adipocyte.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
I J Kozka; A E Clark; J P Reckless; S W Cushman; G W Gould; G D Holman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diabetologia     Volume:  38     ISSN:  0012-186X     ISO Abbreviation:  Diabetologia     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-10-13     Completed Date:  1995-10-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006777     Medline TA:  Diabetologia     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  661-6     Citation Subset:  IM    
Affiliation:
School of Biology and Biochemistry, University of Bath, UK.
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MeSH Terms
Descriptor/Qualifier:
3-O-Methylglucose
Adipocytes / metabolism
Adipose Tissue / drug effects,  metabolism*
Adult
Affinity Labels
Animals
Azides / pharmacology
Biological Transport / drug effects
Blotting, Western
Cells, Cultured
Disaccharides / pharmacology
Female
Glucose / metabolism
Glucose Transporter Type 4
Glycosides
Humans
Insulin / pharmacology*
Kinetics
Male
Methylglucosides / metabolism,  pharmacology
Middle Aged
Monosaccharide Transport Proteins / isolation & purification,  metabolism*
Muscle Proteins*
Propylamines*
Rats
Chemical
Reg. No./Substance:
0/Affinity Labels; 0/Azides; 0/Disaccharides; 0/Glucose Transporter Type 4; 0/Glycosides; 0/Methylglucosides; 0/Monosaccharide Transport Proteins; 0/Muscle Proteins; 0/Propylamines; 0/SLC2A4 protein, human; 0/Slc2a4 protein, rat; 11061-68-0/Insulin; 129461-18-3/2-N-(4-(1-azitrifluoroethyl)benzoyl)-1,3-bis-(mannos-4-yloxy)-2-propylamine; 146-72-5/3-O-Methylglucose; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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