Document Detail


The effects of in vitro pesticide exposures on the phagocytic function of four native Australian freshwater fish.
MedLine Citation:
PMID:  16229903     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There are limited data concerning the lethal and sublethal effects of environmental pollutants on Australian freshwater fish and consequently many of the Australian water quality guidelines are based on data from exotic fish species. This study used a flow cytometric assay to assess the effect of in vitro exposures to commonly used pesticides, on the phagocytic function and cellular composition of head kidney cells from four Australian native fish, i.e. crimson-spotted rainbowfish (Melanotaenia fluviatilis), silver perch (Bidyanus bidyanus), golden perch (Macquaria ambigua) and Murray cod (Maccullochella peelii). Head kidney immune cells were isolated from the four native fish and incubated (1 x 10(6) cells/mL) under previously optimised conditions to measure the phagocytosis of fluorescent-latex beads. These cell cultures were exposed to three classes of pesticides, i.e. the organotins, tributyltin and dibutyltin, the organochlorine endosulfan and the organophosphate chlorpyrifos. The in vitro organotin exposures were highly immunotoxic to head kidney cells from the Australian fish, although there were some differences in immunotoxic responses between species. At the highest concentration (i.e. 10 mg/L), endosulfan exposure resulted in the modulation of phagocytic responses in all species except for silver perch. Chlorpyrifos displayed little immunotoxicity, although there was a dose-dependent reduction in Murray cod lymphocytes. These studies describe the first investigation of the phagocytic response of Australian freshwater fish immunocytes in the presence of environmental pollutants, and will help to determine appropriate ecotoxicity testing for Australian freshwater environments.
Authors:
Andrew J Harford; Kathryn O'Halloran; Paul F A Wright
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-17
Journal Detail:
Title:  Aquatic toxicology (Amsterdam, Netherlands)     Volume:  75     ISSN:  0166-445X     ISO Abbreviation:  Aquat. Toxicol.     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-11-09     Completed Date:  2006-06-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8500246     Medline TA:  Aquat Toxicol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  330-42     Citation Subset:  IM    
Affiliation:
Key Centre for Toxicology, School of Medical Sciences, RMIT-University, P.O. Box 71, Bundoora, Vic. 3083, Australia.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Australia
Dose-Response Relationship, Drug
Fishes / metabolism*
Flow Cytometry
Fresh Water
Kidney / cytology,  drug effects*
Pesticides / toxicity*
Phagocytosis / drug effects*
Species Specificity
Water Pollutants, Chemical / toxicity*
Chemical
Reg. No./Substance:
0/Pesticides; 0/Water Pollutants, Chemical

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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