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The effects of an in vitro exposure to 17β-estradiol and nonylphenol on rainbow trout (Oncorhynchus mykiss) peripheral blood leukocytes.
MedLine Citation:
PMID:  22182679     Owner:  NLM     Status:  Publisher    
While xenoestrogens are routinely detected in the aquatic environment, there is little understanding of the immunotoxicological consequences of exposure to these chemicals in fish, or of the mechanisms through which these effects are mediated. This study was conducted to determine if estrogen receptors (ERs) are present in fish leukocytes and to characterize the effects of 17β-estradiol (E2) and the xenoestrogen nonylphenol (NP) on immune system endpoints in rainbow trout (Oncorhynchus mykiss). Q-PCR was used to confirm that freshly isolated peripheral blood leukocytes (PBLs) express ERs. Following 96-h incubations with E2 or NP (1nM to 10μM), PBL ER transcription was again examined using Q-PCR and lipopolysaccharide (LPS)-stimulated proliferation was assessed using flow cytometry. While the transcription of all four forms of rainbow trout ER was unaffected by treatment with E2 or NP, transcription of ERα1 and ERα2 was down-regulated following LPS stimulation. Both E2 and NP, at concentrations of ≥100nM and 10nM respectively, suppressed leukocyte proliferation. This first report of ERs in rainbow trout PBLs suggests a mechanism through with E2 and other xenoestrogens can modulate immune function. These results highlight the potential for xenoestrogens to impact host resistance to pathogens in wild fish populations.
Lesley K Shelley; Peter S Ross; Christopher J Kennedy
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-13
Journal Detail:
Title:  Comparative biochemistry and physiology. Toxicology & pharmacology : CBP     Volume:  -     ISSN:  1532-0456     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100959500     Medline TA:  Comp Biochem Physiol C Toxicol Pharmacol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Inc.
Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada V5A 1S6.
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