Document Detail

The effects of hyperbaric oxygen treatment on oxidant and antioxidants levels during liver regeneration in rats.
MedLine Citation:
PMID:  15297730     Owner:  NLM     Status:  MEDLINE    
The effects of hyperbaric oxygen (HBO) therapy on oxidant/antioxidant metabolism are controversial and its effects on hepatic regeneration are not known. In this study, we investigated a possible beneficial effect of HBO therapy on oxidant and antioxidants levels during liver regeneration. To conduct this study, seventy percent hepatectomy was performed on forty-eight Spraggue-Dawley rats and the rats were divided into two equal groups: HBO-treated group and untreated group (non-HBO group). We determined the levels of malondialdehyde (MDA), an oxidative stress marker, and the levels of antioxidant enzymes/reagents, including glutathione (GSH), superoxide dismutase (SOD) activity, copper (Cu) and zinc (Zn), in the remnant liver samples. We also measured mitotic index (MI) and proliferating cell nuclear antigen (PCNA) levels to assess the degree of liver regeneration. HBO treatment significantly decreased MDA levels, whereas it increased SOD activity, GSH and Zn levels. In contrast, Cu levels were lower in the HBO-treated livers than the levels in the untreated remnant livers. The effect of HBO treatment may be mediated by the suppression of certain enzymes that are responsible for lipid peroxidation. In addition, HBO treatment may induce the production of antioxidant enzymes/reagents by remnant liver tissues. The HBO-treated rats maintained their body weights but the untreated rats lost body weights. HBO treatment also increased MI and PCNA levels, indicating HBO treatment enhances liver regeneration. These results indicate that HBO treatment has beneficial effects on liver regeneration by decreasing MDA and by increasing antioxidant activities. We therefore suggest that HBO therapy may be useful after liver resection.
Tülin Ayse Ozden; Hafize Uzun; Mehrdad Bohloli; Akin Savas Toklu; Melih Paksoy; Gönül Simsek; Haydar Durak; Halim Issever; Turgut Ipek
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Tohoku journal of experimental medicine     Volume:  203     ISSN:  0040-8727     ISO Abbreviation:  Tohoku J. Exp. Med.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-08-06     Completed Date:  2005-01-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417355     Medline TA:  Tohoku J Exp Med     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  253-65     Citation Subset:  IM    
Institute of Child Health, Trace Element Unit, Istanbul University, Capa, Turkey.
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MeSH Terms
Antioxidants / analysis*,  metabolism
Body Weight
Glutathione Peroxidase / analysis,  metabolism
Hyperbaric Oxygenation*
Liver Regeneration*
Malondialdehyde / analysis,  metabolism
Mitotic Index
Oxidants / analysis*,  metabolism
Proliferating Cell Nuclear Antigen / analysis,  metabolism
Rats, Sprague-Dawley
Superoxide Dismutase / analysis,  metabolism
Thiobarbituric Acid Reactive Substances / analysis
Time Factors
Zinc / analysis,  metabolism
Reg. No./Substance:
0/Antioxidants; 0/Oxidants; 0/Proliferating Cell Nuclear Antigen; 0/Thiobarbituric Acid Reactive Substances; 542-78-9/Malondialdehyde; 7440-66-6/Zinc; EC Peroxidase; EC Dismutase

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