Document Detail

The effects of gossypol on the invasiveness of MAT-LyLu cells and MAT-LyLu cells from the metastasized lungs of MAT-LyLu-bearing Copenhagen rats.
MedLine Citation:
PMID:  11205308     Owner:  NLM     Status:  MEDLINE    
Recently, we isolated a novel subline of the MAT-LyLu cell line from the metastasized lungs of MAT-LyLu-bearing Copenhagen rats (MLL cells). In this study, we compared the MLL cells to the parental MAT-LyLu cells with respect to invasive ability, mRNA expression level for the nm23 metastasis suppressor gene, and response to gossypol (GP), a natural compound with documented antiproliferative and antimetastatic activity, in an in vitro invasion assay. ML cells were isolated from mechanically dissociated metastasized lungs from MAT-LuLu-bearing Copenhagen rats. Comparisons of the invasive ability and steady-state levels of nm23 mRNA between MLL and MAT-LuLu cells were determined by in vitro invasion assay and RT-PCR, respectively. The results show that MLL cells display a higher penetration percentage than MAT-LyLu cells in the in vitro invasion assay. Furthermore, RT-PCR revealed that MLL cells possess lower steady state levels of nm23 mRNA than MAT-LyLu cells, suggesting a molecular basis for the observed differences in in vitro invasive ability. Finally, both MLL and MAT-LyLu cells were susceptible to gossypol, which induced dose-dependent inhibition of invasive activity. These results report the isolation of a novel, more highly invasive subline of the MAT-LyLu cell line that is as susceptible to the inhibitory effects of gossypol as the parental MAT-LyLu cells. The MLL cells, in combination with the parental MAT-LyLu cells, can be valuable tools for investigating the biology and behavior of metastatic cells and their response to chemotherapeutic/preventive agents.
J Jiang; S K Kulp; Y Sugimoto; S Liu; Y C Lin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Anticancer research     Volume:  20     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2000 Nov-Dec
Date Detail:
Created Date:  2001-02-06     Completed Date:  2001-03-01     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  4591-7     Citation Subset:  IM    
Laboratory of Reproductive and Molecular Endocrinology, College of Veterinary Medicine, Ohio State University, 1900 Coffey Road, Columbus, OH 43210, USA.
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MeSH Terms
Dose-Response Relationship, Drug
Genes, Tumor Suppressor
Gossypol / pharmacology*
Monomeric GTP-Binding Proteins / genetics,  metabolism*
NM23 Nucleoside Diphosphate Kinases
Neoplasm Invasiveness / pathology,  prevention & control*
Neoplasm Proteins / genetics,  metabolism*
Neoplasm Transplantation
Nucleoside-Diphosphate Kinase*
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Transcription Factors / genetics,  metabolism*
Tumor Cells, Cultured / drug effects*,  metabolism,  pathology
Grant Support
Reg. No./Substance:
0/NM23 Nucleoside Diphosphate Kinases; 0/Neoplasm Proteins; 0/RNA, Messenger; 0/Transcription Factors; 303-45-7/Gossypol; EC Kinase; EC GTP-Binding Proteins

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