Document Detail

The effects of gamma-hexachlorocyclohexane on amylase secretion and inositol phospholipid metabolism in mouse pancreatic acini.
MedLine Citation:
PMID:  2578827     Owner:  NLM     Status:  MEDLINE    
Dispersed mouse and guinea-pig pancreatic acini were used to examine the effects of the inositol analogue, gamma-hexachlorocyclohexane (lindane) on agonist-stimulated amylase secretion. Secretion from mouse acini in response to carbachol and cholecystokinin octapeptide (CCK-8) was reduced by lindane. Similarly, amylase release from guinea-pig acini stimulated by carbachol was abolished by lindane. These acini, however, still remained responsive to dibutyryl-cAMP with only a slightly diminished secretion to this agent. Inositol phospholipid synthesis and hydrolysis was stimulated in mouse acini by both carbachol and CCK-8. Although hydrolysis of these lipids in response to CCK-8 was reduced by only 18%, stimulation of inositol phospholipid synthesis by either agonist was abolished by lindane. Dose-response curves for inositol phospholipid synthesis stimulated by carbachol and CCK-8 in mouse acini were biphasic and superimposable with those of amylase secretion. In contrast, the dose-response curve for phosphoinositide hydrolysis was sigmoid and clearly separable from that of synthesis. Reducing the external Ca2+ concentration caused the dose-response curves for carbachol- and CCK-8-induced inositol phospholipid synthesis to be displaced to the right, as has been observed for amylase secretion. A23187 was also found to induce amylase secretion and inositol phospholipid synthesis, and both of these responses were inhibited by lindane. Amylase secretion and inositol phospholipid synthesis may, therefore, be closely related events in the exocrine pancreas. Lindane may provide a valuable tool with which to determine the role of inositol phospholipid metabolism in stimulus-response coupling.
M F Crouch; M L Roberts
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  844     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1985 Feb 
Date Detail:
Created Date:  1985-04-04     Completed Date:  1985-04-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  149-57     Citation Subset:  IM    
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MeSH Terms
Amylases / metabolism*
Bucladesine / pharmacology
Calcimycin / pharmacology
Dose-Response Relationship, Drug
Guinea Pigs
Inositol / metabolism
Lindane / pharmacology*
Pancreas / drug effects*
Phosphatidylinositols / metabolism*
Quinuclidinyl Benzilate / metabolism
Sincalide / pharmacology
Reg. No./Substance:
0/Phosphatidylinositols; 25126-32-3/Sincalide; 362-74-3/Bucladesine; 52665-69-7/Calcimycin; 58-89-9/Lindane; 6581-06-2/Quinuclidinyl Benzilate; 6917-35-7/Inositol; EC 3.2.1.-/Amylases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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