| The effects of estrogens and antiestrogens on hormone-responsive human breast cancer in long-term tissue culture. | |
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MedLine Citation:
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PMID: 1000504 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have established or characterized six lines of human breast cancer maintained in long-term tissue culture for at least 1 year and have examined these lines for estrogen responsiveness. One of these cell lines, MCF-7, shows marked stimulation of macromolecular synthesis and cell division with physiological concentrations of estradiol. Antiestrogens are strongly inhibitory, and at concentrations greater than 3 X 10(-7) M they kill cells. Antiestrogen effects are prevented by simultaneous treatment with estradiol or reversed by addition of estradiol to cells incubated in antiestrogen. Responsive cell lines contain high-affinity specific estradiol receptors. Antiestrogens compete with estradiol for these receptors but have a lower apparent affinity for the receptor than estrogens. Stimulation of cells by estrogens is biphasic, with inhibition and cell death at concentrations of 17beta-estradiol or diethylstilbestrol exceeding 10(-7) M. Killing by high concentrations of estrogen is probably a nonspecific effect in that we observe this response with 17alpha-estradiol at equivalent concentrations and in the otherwise unresponsive cells that contain no estrogen receptor sites. |
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Authors:
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M Lippman; G Bolan; K Huff |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cancer research Volume: 36 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1976 Dec |
Date Detail:
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Created Date: 1977-02-16 Completed Date: 1977-02-16 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4595-601 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Binding Sites Binding, Competitive Breast Neoplasms / metabolism*, pathology Cell Division / drug effects Cell Line Cells, Cultured DNA, Neoplasm / biosynthesis Diethylstilbestrol / pharmacology Dose-Response Relationship, Drug Estradiol / pharmacology Estrogens / antagonists & inhibitors*, pharmacology* Female Humans Nitromifene / pharmacology RNA, Neoplasm / biosynthesis Receptors, Estrogen* Tamoxifen / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 0/Estrogens; 0/RNA, Neoplasm; 0/Receptors, Estrogen; 10448-84-7/Nitromifene; 10540-29-1/Tamoxifen; 50-28-2/Estradiol; 56-53-1/Diethylstilbestrol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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