Document Detail

The effects of estradiol on estrogen receptor and glutamate transporter expression in organotypic hippocampal cultures exposed to oxygen--glucose deprivation.
MedLine Citation:
PMID:  16758356     Owner:  NLM     Status:  MEDLINE    
The molecular basis of estrogen-mediated neuroprotection against brain ischemia remains unclear. In the present study, we investigated changes in expression of estrogen receptors (ERs) alpha and beta and excitatory amino acid transporters (EAAT) 1 and 2 in rat organotypic hippocampal slice cultures treated with estradiol and subsequently exposed to oxygen--glucose deprivation (OGD). Pretreatment with 17beta-estradiol (10 nM) for 7 days protected the CA1 area of hippocampus against OGD (60 min), reducing cellular injury by 46% compared to the vehicle control group. Levels of ERalpha protein were significantly reduced by 20% after OGD in both vehicle- and estradiol-treated cultures, whereas ERbeta was significantly up-regulated by 25% in the estradiol-treated cultures. In contrast, EAAT1 and EAAT2 levels were unchanged in response to estradiol treatment in this model of OGD. These findings suggest that estrogen-induced neuroprotection against ischemia might involve regulation of ERbeta and, consequently, of the genes influenced by this receptor.
Helena Cimarosti; Ross D O'Shea; Nicole M Jones; Ana Paula Horn; Fabrício Simão; Lauren L Zamin; Melissa Nassif; Rudimar Frozza; Carlos Alexandre Netto; Philip M Beart; Christianne Salbego
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-09
Journal Detail:
Title:  Neurochemical research     Volume:  31     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-06-07     Completed Date:  2007-03-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  483-90     Citation Subset:  IM    
Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Allegre, Brazil .
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MeSH Terms
Cells, Cultured
Estradiol / metabolism*
Estrogen Receptor alpha / metabolism*
Estrogen Receptor beta / metabolism*
Excitatory Amino Acid Transporter 1 / metabolism*
Excitatory Amino Acid Transporter 2 / metabolism*
Glucose / metabolism*
Hippocampus / cytology*,  metabolism
Rats, Wistar
Reg. No./Substance:
0/Estrogen Receptor alpha; 0/Estrogen Receptor beta; 0/Excitatory Amino Acid Transporter 1; 0/Excitatory Amino Acid Transporter 2; 0/Slc1a2 protein, rat; 0/Slc1a3 protein, rat; 50-28-2/Estradiol; 50-99-7/Glucose

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