Document Detail


The effects of erythrocyte deformability upon hematocrit assessed by the conductance method.
MedLine Citation:
PMID:  19336847     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A comparative study of centrifugation and conductance methods for the estimation of cell volume fraction (phi) was performed to examine whether the strong forces exerted upon erythrocytes during centrifugation affect their volume, and the results are discussed in terms of erythrocyte deformability. Rabbit erythrocytes of four shapes (spherocytes, echinocytes, stomatocyte-like enlarged erythrocytes and discocytes) were prepared by controlling the pH of the suspending media. The packed cell volumes of the suspensions were measured by standard hematocrit determination methods using centrifugation in capillary tubes. Simultaneously, the same suspensions and their supernatants were used in dielectric spectroscopy measurements, and the low-frequency limits of their conductivities were used for the numerical estimation of phi. The hematocrit values of spherocytes and echinocytes were markedly less than the volume fractions obtained by the conductance method. Namely, the centrifugation reduced the cell volume. For enlarged erythrocytes and discocytes, however, the reduction of cell volume was not observed. These findings showed that phi obtained by the centrifugation method can be greatly affected by the deformability of the cells, but the level of the effect depends on the cell types. Consequently, phi obtained by the centrifugation method should be carefully interpreted.
Authors:
Yoshihito Hayashi; Yoichi Katsumoto; Ikuya Oshige; Shinji Omori; Akio Yasuda; Koji Asami
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Publication Detail:
Type:  Journal Article     Date:  2009-04-01
Journal Detail:
Title:  Physics in medicine and biology     Volume:  54     ISSN:  0031-9155     ISO Abbreviation:  Phys Med Biol     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-07     Completed Date:  2009-06-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401220     Medline TA:  Phys Med Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2395-405     Citation Subset:  IM    
Affiliation:
Life Science Laboratory, Advanced Materials Laboratories, Sony Corporation, Sony Bioinformatics Center, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8510, Japan. Yoshihito.Hayashi@jp.sony.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Shape / drug effects
Cell Size / drug effects
Centrifugation
Electric Conductivity*
Erythrocyte Deformability* / drug effects
Erythrocyte Volume / drug effects
Glutaral / pharmacology
Hematocrit
Hydrogen-Ion Concentration
Microscopy
Models, Biological
Rabbits
Reproducibility of Results
Chemical
Reg. No./Substance:
111-30-8/Glutaral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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