| The effects of endothelial factor inhibition on the time course of responses of isolated rat coronary arteries to intraluminal flow. | |
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MedLine Citation:
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PMID: 17337908 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aims of this study were to investigate, for the first time, the effects of endothelial factor inhibition on both the magnitude and dynamics of the response of isolated small coronary arteries to intraluminal flow. Isolated rat coronary arteries were mounted on a pressure myograph and left to develop myogenic tone. Flow was introduced and maintained until stable diameters were attained. Dilatory responses were observed which were maximal at low flow rates (5-10 microl/min) and thus shear stresses (1-2 dyn/cm(2)). These responses were transient in nature. Transient dilations were also observed upon cessation of flow. All responses (to 5 microl/min) were endothelium dependent and were completely abolished by addition of charybdotoxin (100 nM) and apamin (100-500 nM) suggesting an important role for a hyperpolarizing mechanism most likely involving an endothelium-derived hyperpolarizing factor. However, inhibitors of nitric oxide synthase (L-NNA; 100 microM) or cyclo-oxygenase (indomethacin; 10 microM) also modulated the response causing an increase and decrease in maximum vasodilation, respectively. By examining the time course we showed that both agents also made the response significantly more transient in nature. These results show that inhibition of endothelial factor pathways can influence both the magnitude and dynamics of the response of isolated rat coronary arteries to flow. |
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Authors:
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May Azzawi; Clare Austin |
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Publication Detail:
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Type: In Vitro; Journal Article Date: 2007-02-28 |
Journal Detail:
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Title: Journal of vascular research Volume: 44 ISSN: 1018-1172 ISO Abbreviation: J. Vasc. Res. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-04-13 Completed Date: 2007-05-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9206092 Medline TA: J Vasc Res Country: Switzerland |
Other Details:
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Languages: eng Pagination: 223-33 Citation Subset: IM |
Affiliation:
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Smooth Muscle Physiology Group, Division of Cardiovascular and Endocrine Sciences, Core Technology Facility, University of Manchester, Manchester, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apamin / pharmacology Biological Factors / metabolism Charybdotoxin / pharmacology Coronary Vessels / drug effects, metabolism* Cyclooxygenase Inhibitors / pharmacology Endothelium, Vascular / drug effects, metabolism* Indomethacin / pharmacology Male Myography Nitric Oxide / metabolism Nitric Oxide Synthase / antagonists & inhibitors Nitroarginine / pharmacology Potassium Channel Blockers / pharmacology Pressure Prostaglandins / metabolism Pulsatile Flow Rats Rats, Wistar Stress, Mechanical Time Factors Vasodilation* / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Biological Factors; 0/Cyclooxygenase Inhibitors; 0/Potassium Channel Blockers; 0/Prostaglandins; 0/endothelium-dependent hyperpolarization factor; 10102-43-9/Nitric Oxide; 115422-61-2/Charybdotoxin; 2149-70-4/Nitroarginine; 24345-16-2/Apamin; 53-86-1/Indomethacin; EC 1.14.13.39/Nitric Oxide Synthase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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