| The effects of electromagnetic pulses (EMP) on the bioactivity of insulin and a preliminary study of mechanism. | |
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MedLine Citation:
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PMID: 20070212 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To investigate the effects of electromagnetic pulse (EMP) exposure on the bioactivity of insulin and a preliminary mechanism for these effects. MATERIALS AND METHODS: A tapered parallel plate Gigahertz Transverse Electromagnetic (GTEM) cell with a flared rectangular coaxial transmission line was used to expose the insulin solution to EMP. Concurrent sham-exposed insulin solutions were used as a control. The effect of EMP-exposed insulin on fasting blood glucose levels of type I diabetes model mice, the effect of EMP on binding affinity between insulin and its receptor and the effect of EMP on insulin's fluorescence intensity were detected, respectively. RESULTS: (i) After EMP exposure, compared with sham-exposed insulin, the bioactivity of insulin in decreasing fasting blood glucose levels in type I diabetes model mice was reduced significantly (p = 0.023). (ii) Compared with sham-exposed insulin group, the percentage fluorescein isothiocyannate (FITC) labelling of HL-7702 cells was significantly reduced in the EMP-exposed insulin group (22.7-13.8%, respectively). (iii) Compared with sham-exposed insulin, the fluorescence intensity was significantly reduced in EMP-exposed insulin (p < 0.001). CONCLUSIONS: EMP exposure significantly decreased the bioactivity of insulin to reduce the blood glucose levels in type I diabetic mice. This could be due to a decreased binding affinity between insulin and its receptor. This mechanism could involve an alteration of insulin's' conformation caused by EMP exposure. |
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Authors:
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Yong Bin Chen; Jing Li; Yuhong Qi; Xia Miao; Yongchun Zhou; Dongqing Ren; G Z Guo |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of radiation biology Volume: 86 ISSN: 1362-3095 ISO Abbreviation: Int. J. Radiat. Biol. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-01-14 Completed Date: 2010-01-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8809243 Medline TA: Int J Radiat Biol Country: England |
Other Details:
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Languages: eng Pagination: 22-6 Citation Subset: IM; S |
Affiliation:
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Department of Radiation Medicine, Fourth Military Medical University, Xi'an, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Glucose / analysis Cell Line Diabetes Mellitus, Type 1 / blood, drug therapy Electromagnetic Fields* Humans Insulin / metabolism, pharmacology* Male Mice Mice, Inbred BALB C |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 11061-68-0/Insulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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