| The effects of a duplication in the ovine growth hormone (GH) gene on GH expression in the pituitaries of ram lambs from lean and fat-selected sheep lines. | |
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MedLine Citation:
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PMID: 8985666 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Growth hormone (GH) gene expression was investigated in pituitaries of 14- to 15-month-old ram lambs from flocks selected for high (fat) or low (lean) back fat depth, which were also homozygous for a single GH gene allele, heterozygous or homozygous for a duplication in the GH gene. The pituitaries of lean sheep of all three GH genotypes were significantly heavier than those of fat sheep, but there were no pituitary weight differences between GH genotypes. No significant lean-fat selection line- or GH genotype-specific differences were measured in pituitary GH concentration. However there was a significant increase (P < 0.01) in the total pituitary content of GH in lean compared with fat animals and a significant interaction between GH genotype and lean-fat selection line (P < 0.05) was noted for GH content. No significant differences were measured in the relative concentration of GH mRNA, suggesting that the ratio of GH mRNA per mg total cellular RNA remained constant across lean-fat selection line and GH genotype. We conclude that the pituitary glands of Coopworth sheep selected for low backfat depth (lean) are bigger and have an increased GH content, but appear to contain similar concentrations of GH mRNA and immunoreactive GH as the pituitaries of fat sheep. The presence of the GH gene duplication in sheep has little measurable effect on the expression and storage of GH in the pituitary. |
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Authors:
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J S Fleming; J M Suttie; G W Montgomery; J Gunn; S K Stuart; R P Littlejohn; E Gootwine |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Domestic animal endocrinology Volume: 14 ISSN: 0739-7240 ISO Abbreviation: Domest. Anim. Endocrinol. Publication Date: 1997 Jan |
Date Detail:
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Created Date: 1997-03-20 Completed Date: 1997-03-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8505191 Medline TA: Domest Anim Endocrinol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 17-24 Citation Subset: IM |
Affiliation:
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AgResearch, Invermay Agricultural Centre, Mosgiel, New Zealand. jean.fleming@stonebow.otago.ac.nz |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Animals Blotting, Northern / methods, veterinary Body Weight / genetics*, physiology Gene Expression Regulation Genotype Growth Hormone / analysis*, genetics*, metabolism Heterozygote Homozygote Male Multigene Family* Obesity / metabolism, physiopathology, veterinary* Pituitary Gland / chemistry*, metabolism RNA, Messenger / analysis, genetics, metabolism Sheep / genetics*, metabolism*, physiology |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 9002-72-6/Growth Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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