Document Detail

The effects of different intraocular irrigating solutions on the apoptosis of cultured RPE cells.
MedLine Citation:
PMID:  12898278     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To investigate the effect on porcine retinal pigment epithelial (RPE) cells of modified intraocular irrigating solutions compared with BSS and BSS plus. METHODS: Confluent cultures of RPE cells were incubated in experimental intraocular irrigating solutions. The cells were then examined for breakdown of DNA by the TUNEL procedure. Fragmentation of the DNA from cells was also confirmed by flow cytometry. RESULTS: Most of the experimentally, treated cells exhibited a shrunken appearance for up to 72 h. There was a steady increase in the number of cells labeled by the TUNEL method in three solutions with time. The data demonstrated that the influence of solutions on the percentage of RPE cell nuclei that gave a clear positive TUNEL stain was, in ascending order: modified solution>BSS>BSS plus. Of the three solutions tested, BSS plus showed least apoptosis. CONCLUSIONS: It is suggested that BSS plus is less harmful to RPE cells than the other solutions tested. Introduction of other adjunctive solutions of antibiotics, mydriatics, miotics, and steroid, resulting in an altered electrolyte balance, pH, or osmolality in the solution, may compromise the safety and efficacy of a properly formulated and packaged solution.
Chunyue Yin; Xiaoxin Li
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2003-07-25
Journal Detail:
Title:  Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie     Volume:  241     ISSN:  0721-832X     ISO Abbreviation:  Graefes Arch. Clin. Exp. Ophthalmol.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-11-07     Completed Date:  2004-01-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8205248     Medline TA:  Graefes Arch Clin Exp Ophthalmol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  834-9     Citation Subset:  IM    
Department of Ophthalmology, The Second Teaching Hospital (People's Hospital), No 11, Xizhimen South St, Xicheng District, 100044 Beijing, PR China.
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MeSH Terms
Acetates / pharmacology*
Apoptosis / drug effects*
Bicarbonates / pharmacology*
Cell Count
Cells, Cultured
DNA Fragmentation
Drug Combinations
Flow Cytometry
Glutathione / pharmacology*
In Situ Nick-End Labeling
Minerals / pharmacology*
Ophthalmic Solutions
Pigment Epithelium of Eye / drug effects*,  pathology*
Sodium Chloride / pharmacology*
Reg. No./Substance:
0/Acetates; 0/BSS solution; 0/Bicarbonates; 0/Drug Combinations; 0/Minerals; 0/Ophthalmic Solutions; 0/glutathione-bicarbonate-Ringer solution; 70-18-8/Glutathione; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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