Document Detail

The effects of continuous and discontinuous groove edges on cell shape and alignment.
MedLine Citation:
PMID:  12878169     Owner:  NLM     Status:  MEDLINE    
Nanofabricated model surfaces and digital image analysis of cell shape were used to address the importance of a continuous sharp edge in the alignment of cells to shallow surface grooves. The grooved model surfaces had either continuous or discontinuous edges of various depths (40-400 nm) but identical surface chemistry and groove/ridge dimensions (15 microm wide). Epithelial cells were cultured on the model surfaces for 10 and 24 h. Fluorescence microscopy combined with image analysis were used to quantify cell area and alignment and to make cell shape classifications of individual cells. The degrees of alignment of cells and the percentages of elongated cell classes increased with groove depth on samples with continuous grooves. Two main differences, with regard to cell response, were observed between the continuous and discontinuous grooved surfaces. First, significantly fewer cells aligned to surface grooves with discontinuous edges than to grooves with continuous edges. Second, there were lower percentages of the elongated cell classes on discontinuous grooves than on continuous ones. We concluded that grooved surfaces with continuous edges are more potent in aligning and inducing elongated cells. The results from the present study suggest that a mechanism of alignment involving orientation along a continuous edge is likely.
Ann-Sofie Andersson; Petra Olsson; Ulf Lidberg; Duncan Sutherland
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  288     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-07-24     Completed Date:  2003-09-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  177-88     Citation Subset:  IM    
Department of Applied Physics, Chalmers University of Technology, SE-412 96 Göteborg, Sweden.
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MeSH Terms
Cell Adhesion
Cell Line
Cell Polarity
Cell Size
Epithelial Cells / cytology*
Imaging, Three-Dimensional
Microscopy, Fluorescence
Surface Properties

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