Document Detail

The effects of coexisting lipids on the action of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C toward liposomal substrate.
MedLine Citation:
PMID:  1799944     Owner:  NLM     Status:  MEDLINE    
The hydrolytic activity of phosphatidylinositol (PI)-specific phospholipase C (PI-PLC) from Bacillus thuringiensis was studied in detail toward mixed liposomes consisting of PI and one of other phospholipids and cholesterol. Among PI-liposomes, small unilamellar vesicles (SUV) were the most sensitive to PI-PLC; the enzymatic hydrolysis of PI in SUV was not less than 10-fold that in large unilamellar vesicles (LUV) or in multilamellar vesicles (MLV). Thus, in a survey of the effects of coexisting lipids on PI-PLC activity, PI-SUV was used. Phosphatidylcholine (PC) was stimulative for the enzyme activity toward PI-SUV at any molar ratio of PC to PI. Also, the effects of the addition of sphingomyelin (SM), phosphatidylethanolamine (PE) and cholesterol on the enzymatic hydrolysis of PI were studied in detail on the basis of concentration of total lipids or PI.
T Kume; R Taguchi; H Ikezawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chemical & pharmaceutical bulletin     Volume:  39     ISSN:  0009-2363     ISO Abbreviation:  Chem. Pharm. Bull.     Publication Date:  1991 Nov 
Date Detail:
Created Date:  1992-04-20     Completed Date:  1992-04-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0377775     Medline TA:  Chem Pharm Bull (Tokyo)     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  2980-3     Citation Subset:  IM    
Faculty of Pharmaceutical Sciences, Nagaya City University, Aichi, Japan.
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MeSH Terms
Bacillus thuringiensis / enzymology*
Cholesterol / chemistry
Lipids / pharmacology*
Phosphatidylinositols / metabolism
Type C Phospholipases / metabolism*
Reg. No./Substance:
0/Lipids; 0/Liposomes; 0/Phosphatidylinositols; 57-88-5/Cholesterol; EC 3.1.4.-/Type C Phospholipases

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