| The effects of chemical enucleation combined with whole cell intracytoplasmic injection on panda-rabbit interspecies nuclear transfer. | |
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MedLine Citation:
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PMID: 15751540 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Conventional methods of somatic cell nuclear transfer either by electrofusion or direct nucleus injection have very low efficiency in animal cloning, especially interspecies cloning. To increase the efficiency of interspecies somatic cell nuclear transfer, in the present study we introduced a method of whole cell intracytoplasmic injection (WCICI) combined with chemical enucleation into panda-rabbit nuclear transfer and assessed the effects of this method on the enucleation rate of rabbit oocytes and the in vitro development and spindle structures of giant panda-rabbit reconstructed embryos. Our results demonstrated that chemical enucleation can be used in rabbit oocytes and the optimal enucleation result can be obtained. When we compared the rates of cleavage and blastocyst formation of subzonal injection (SUZI) and WCICI using chemically enucleated rabbit oocytes as cytoplasm recipients, the rates in the WCICI group were higher than those in the SUZI group, but there was no statistically siginificant difference (p > 0.05) between the two methods. The microtubule structures of rabbit oocytes enucleated by chemicals and giant panda-rabbit embryos reconstructed by WCICI combined with chemical enucleation were normal. Therefore the present study suggests that WCICI combined with chemical enucleation can provide an efficient and less labor-intensive protocol of interspecies somatic cell nuclear transfer for producing giant panda cloned embryos. |
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Authors:
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Man-Xi Jiang; Cai-Xia Yang; Li-Sheng Zhang; Yue-Liang Zheng; Shu-Zhen Liu; Qing-Yuan Sun; Da-Yuan Chen |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Zygote (Cambridge, England) Volume: 12 ISSN: 0967-1994 ISO Abbreviation: Zygote Publication Date: 2004 Nov |
Date Detail:
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Created Date: 2005-03-08 Completed Date: 2005-06-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9309124 Medline TA: Zygote Country: England |
Other Details:
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Languages: eng Pagination: 315-20 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcimycin Cell Nucleus / drug effects Cloning, Organism / methods* Cytochalasins Demecolcine / pharmacology Embryonic Development / physiology* Ionomycin Male Microinjections / methods Microscopy, Confocal Microtubules / drug effects Nuclear Transfer Techniques* Oocytes / cytology, drug effects Rabbits Transplantation, Heterologous Ursidae / embryology* |
| Chemical | |
Reg. No./Substance:
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0/Cytochalasins; 477-30-5/Demecolcine; 52665-69-7/Calcimycin; 56092-81-0/Ionomycin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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