| The effects of buthionine sulfoximine, diethyldithiocarbamate or 3-amino-1,2,4-triazole on propyl gallate-treated HeLa cells in relation to cell growth, reactive oxygen species and glutathione. | |
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MedLine Citation:
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PMID: 19578799 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Propyl gallate (PG) as a synthetic antioxidant is widely used in processed food and medicinal preparations. It also exerts a variety of effects on tissue and cell functions. In the present study, we investigated the effects of L-buthionine sulfoximine (BSO, an inhibitor of GSH synthesis), diethyldithiocarbamate (DDC, an inhibitor of Cu/Zn-SOD) or 3-amino-1,2,4-triazole (AT, an inhibitor of catalase) on PG-treated HeLa cells in relation to cell growth, reactive oxygen species (ROS) and glutathione (GSH). Treatment with PG induced growth inhibition, the loss of mitochondrial membrane potential [MMP (DeltaPsim)] and apoptosis in HeLa cells. ROS levels including O2.- were increased or decreased in PG-treated HeLa cells depending on the incubation times. PG caused depletion in GSH content in HeLa cells. While BSO enhanced the growth inhibition of PG-treated HeLa cells at 4 h, DDC and AT did not. All the agents down-regulated MMP (DeltaPsim) levels in PG-treated cells. Although BSO, DDC or AT slightly increased ROS or O2.- levels in PG-treated cells at 1 h, these enhancements of ROS did not intensify apoptosis in these cells. In addition, BSO, DDC or AT slightly reduced GSH level in PG-treated HeLa cells at 1 h, but this reduction did not affect cell death of HeLa. Furthermore, PG induced a G1 phase arrest of the cell cycle. BSO, DDC or AT significantly inhibited the G1 phase arrest in PG-treated cells. Conclusively, the changes of ROS and GSH levels by BSO, DDC or AT in PG-treated HeLa cells did not strongly affect the cell growth and death. |
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Authors:
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Yong Hwan Han; Hwa Jin Moon; Bo Ra You; Sung Zoo Kim; Suhn Hee Kim; Woo Hyun Park |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of molecular medicine Volume: 24 ISSN: 1107-3756 ISO Abbreviation: Int. J. Mol. Med. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-06 Completed Date: 2009-09-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9810955 Medline TA: Int J Mol Med Country: Greece |
Other Details:
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Languages: eng Pagination: 261-8 Citation Subset: IM |
Affiliation:
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Department of Physiology, Medical School, Center for Healthcare Technology Development, Institute for Medical Sciences, Chonbuk National University, JeonJu 561-180, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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pharmacology Apoptosis / drug effects Buthionine Sulfoximine / pharmacology Catalase / antagonists & inhibitors, metabolism Cell Cycle / drug effects Cell Proliferation / drug effects* Ditiocarb / pharmacology Enzyme Inhibitors / pharmacology* Glutathione / antagonists & inhibitors, metabolism* Hela Cells Humans Membrane Potential, Mitochondrial / drug effects Propyl Gallate / pharmacology* Reactive Oxygen Species / metabolism* Superoxide Dismutase / antagonists & inhibitors, metabolism Time Factors Triazoles / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Enzyme Inhibitors; 0/Reactive Oxygen Species; 0/Triazoles; 121-79-9/Propyl Gallate; 147-84-2/Ditiocarb; 288-88-0/1,2,4-triazole; 5072-26-4/Buthionine Sulfoximine; 70-18-8/Glutathione; EC 1.11.1.6/Catalase; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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