| The effects of branched-chain amino acid interactions on growth performance, blood metabolites, enzyme kinetics and transcriptomics in weaned pigs. | |
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MedLine Citation:
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PMID: 20196890 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The impact of excess dietary leucine (Leu) was studied in two growth assays with pigs (8-25 kg). In each trial, forty-eight pigs were allotted to one of six dietary groups. The dietary Leu supply increased from treatment L100 to L200 (three increments). To guarantee that interactions between the branched-chain amino acids (BCAA) were not cushioned either surpluses of isoleucine (Ile, expt 1) or valine (Val; expt 2) were avoided. In the fifth treatment, the effects of a simultaneous excess of Leu and Val (expt 1), or of Leu and Ile (expt 2) were investigated. The sixth treatment was a positive control. An increase in dietary Leu decreased growth performance, and increased plasma Leu and serum alpha-keto-isocaproate levels in a linear, dose-dependent manner. Levels of plasma Ile and Val, and of serum alpha-keto-beta-methylvalerate and alpha-keto-isovalerate, indicated increased catabolism. Linear increases in the activity of basal branched-chain alpha-keto acid dehydrogenase in the liver confirmed these findings. No major alterations occurred in the mRNA of branched-chain amino acid catabolism genes. In liver tissue from expt 2, however, the mRNA levels of growth hormone receptor, insulin-like growth factor acid labile subunit and insulin-like growth factor 1 decreased significantly with increasing dietary Leu. In conclusion, excess dietary Leu increased the catabolism of BCAA mainly through posttranscriptional mechanisms. The impact of excess Leu on the growth hormone--insulin-like growth factor-1 axis requires further investigation. |
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Authors:
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Markus Karl Wiltafsky; Michael Walter Pfaffl; Franz Xaver Roth |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-03 |
Journal Detail:
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Title: The British journal of nutrition Volume: 103 ISSN: 1475-2662 ISO Abbreviation: Br. J. Nutr. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-08 Completed Date: 2010-04-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372547 Medline TA: Br J Nutr Country: England |
Other Details:
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Languages: eng Pagination: 964-76 Citation Subset: IM |
Affiliation:
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Department f?r Tierwissenschaften, Lehrstuhl f?r Tierern?hrung, Technische Universit?t M?nchen, Hochfeldweg 6, Freising D-85350, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
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metabolism* Amino Acids, Branched-Chain / blood, metabolism, pharmacology* Analysis of Variance Animals Diet* Dose-Response Relationship, Drug Female Gene Expression Profiling Isoleucine / blood, metabolism, pharmacology Keto Acids / blood* Leucine / blood, metabolism, pharmacology* Linear Models Liver / metabolism Metabolism / drug effects RNA, Messenger / metabolism Receptors, Somatotropin / genetics, metabolism Somatomedins / genetics, metabolism Swine / growth & development*, metabolism Valine / blood, metabolism, pharmacology Weaning |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids, Branched-Chain; 0/Keto Acids; 0/RNA, Messenger; 0/Receptors, Somatotropin; 0/Somatomedins; 61-90-5/Leucine; 7004-03-7/Valine; 73-32-5/Isoleucine; EC 1.2.4.4/3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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