Document Detail


The effects of blocking the actions of estrogen and progesterone on the rates of proliferation and apoptosis of cervical epithelial and stromal cells during the second half of pregnancy in rats.
MedLine Citation:
PMID:  15972881     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Serum levels of the ovarian hormones relaxin, estrogen, and progesterone are elevated during the second half of 23-day rat pregnancy when dramatic growth of the cervix occurs. Recently, we demonstrated that relaxin contributes to cervical growth by both promoting cell proliferation and inhibiting apoptosis of cervical cells during late pregnancy. The objective of this study was to determine the influence of estrogen and progesterone on the rates of proliferation and apoptosis of cervical cells at 3-day intervals during the second half of rat pregnancy. The actions of estrogen and progesterone were blocked with s.c. injections of estrogen antagonist ICI 182,780 and progesterone antagonist RU486, respectively. To evaluate cell proliferation, 5'-bromo-2'-deoxyuridine was injected s.c. 8 h before cervixes were collected. Terminal deoxynucleotidyl transferase-mediated deoxyuridine 5'-triphosphate nick end-labeling was used to detect apoptotic cells. Proliferating and apoptotic cells were identified by immunohistochemistry, and the rates at which these processes occurred were determined by morphometric analysis. Blocking the actions of estrogen and progesterone decreased the rates of proliferation and increased the rates of apoptosis of both cervical epithelial and stromal cells during late pregnancy. However, blocking the actions of progesterone had the opposite effects on apoptosis of both cervical epithelial and stromal cells during the middle of pregnancy. In conclusion, this study provides evidence that estrogen and progesterone, like relaxin, contribute to the increase in the cervical cell content during late pregnancy by promoting proliferation and inhibiting apoptosis of cervical cells.
Authors:
Hyung-Yul Lee; O David Sherwood
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.     Date:  2005-06-22
Journal Detail:
Title:  Biology of reproduction     Volume:  73     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-21     Completed Date:  2006-01-23     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  790-7     Citation Subset:  IM    
Affiliation:
Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, 61801, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects,  physiology*
Cell Proliferation
Cervix Uteri / cytology*
Epithelial Cells / drug effects
Estradiol / analogs & derivatives,  pharmacology
Estrogen Antagonists / pharmacology
Estrogens / blood,  metabolism*
Female
Hormone Antagonists / pharmacology*
Mifepristone / pharmacology
Pregnancy
Pregnancy, Animal
Progesterone / blood,  metabolism*
Rats
Rats, Sprague-Dawley
Relaxin / metabolism
Stromal Cells
Grant Support
ID/Acronym/Agency:
R01 HD40448/HD/NICHD NIH HHS; T32 GM07143/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Estrogen Antagonists; 0/Estrogens; 0/Hormone Antagonists; 22X328QOC4/fulvestrant; 50-28-2/Estradiol; 57-83-0/Progesterone; 84371-65-3/Mifepristone; 9002-69-1/Relaxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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