| The effects of binge alcohol exposure in the 2nd trimester on the estimated density of cerebral microvessels in near-term fetal sheep. | |
| | |
MedLine Citation:
|
PMID: 18657528 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Heavy fetal alcohol exposure is associated with a spectrum of neurological abnormalities, although the mechanism of injury is largely unknown. We previously reported attenuated cerebral blood flow response to hypoxia in fetal and newborn sheep which were exposed to alcohol earlier in pregnancy. One possible mechanism for this effect of alcohol on the developing cerebral vasculature is a decrease in cerebral microvessel density, similar to its effect on developing neurons. Therefore, we tested the hypothesis that prenatal alcohol exposure decreases cerebral microvessel density. Pregnant ewes received intravenous infusions of ethanol or saline during days 60-84 of gestation (term=150 days) and at 125 days of gestation we obtained the fetal brains for study. We immunohistochemically labeled vessels of the left cerebral forebrain hemispheres with antibody to endothelial nitric oxide synthase and then obtained unbiased stereological estimates of cerebral microvessel density using a modified optical fractionator method. We studied 20 fetal brains of which 9 were alcohol-exposed, 11 were saline-controls, and all were products of a twin gestation. Although brain and body weights were not different between groups, the alcohol-exposed group had significantly lower brain weight as a percentage of body weight. Estimates of cerebral microvessel density were not significantly different between alcohol-exposed and saline-control groups: 12.7+/-8.7 and 9.1+/-2.8 microvessels per mm(3), respectively (mean+/-SD, p=0.32). Since there is no change in estimated cerebral microvessel density after prenatal alcohol exposure, we conclude that decreased cerebral microvessel density is not a likely explanation for attenuated cerebral blood flow in response to hypoxia. |
| | |
Authors:
|
Katherine E Simon; Robin L Mondares; Donald E Born; Christine A Gleason |
Related Documents
:
|
10227338 - Inhibitory effects of azelastine hydrochloride in alcohol-induced asthma. 10064168 - Corpus callosum atrophy and cerebral blood flow in chronic alcoholics. 8156418 - Effect of dietary contents of cholecalciferol, 1 alpha,25-dihydroxycholecalciferol and ... 9548988 - Estimating the time between drinking and death from tissue distribution patterns of eth... 12751638 - Effect of geometric structure of flow promoting agents on the flow properties of pharma... 2676188 - Investigation of the performance of two types of the doppler catheter in vitro. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-07-15 |
Journal Detail:
|
Title: Brain research Volume: 1231 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 2008 Sep |
Date Detail:
|
Created Date: 2008-09-08 Completed Date: 2009-01-07 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0045503 Medline TA: Brain Res Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 75-80 Citation Subset: IM |
Affiliation:
|
Department of Pediatrics, University of Washington, Seattle, WA, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alcohol-Induced Disorders, Nervous System
/
pathology*,
physiopathology Alcoholism / complications Animals Arterioles / abnormalities, drug effects, physiopathology Central Nervous System Depressants / toxicity Cerebral Arteries / abnormalities*, drug effects*, physiopathology Cerebrovascular Circulation / drug effects Disease Models, Animal Ethanol / toxicity Female Fetal Alcohol Syndrome / pathology*, physiopathology Hypoxia, Brain / physiopathology Male Microcirculation / drug effects* Neovascularization, Physiologic / drug effects Nitric Oxide Synthase Type III / drug effects, metabolism Organ Size / drug effects Pregnancy Pregnancy Trimester, Second / drug effects Prosencephalon / blood supply, drug effects, physiopathology Sheep |
| Grant Support | |
ID/Acronym/Agency:
|
R01 AA 12403/AA/NIAAA NIH HHS; R01 AA012403-01/AA/NIAAA NIH HHS; R01 AA012403-02/AA/NIAAA NIH HHS; R01 AA012403-02S1/AA/NIAAA NIH HHS; R01 AA012403-03/AA/NIAAA NIH HHS; R01 AA012403-04/AA/NIAAA NIH HHS; R01 AA012403-05/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Central Nervous System Depressants; 64-17-5/Ethanol; EC 1.14.13.39/Nitric Oxide Synthase Type III |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Behavioral and electrophysiological studies in rats with cisplatin-induced chemoneuropathy.
Next Document: Hippocampal RAGE immunoreactivity in early and advanced Alzheimer's disease.