Document Detail


The effects of autologous bone marrow mesenchymal stem cell arterial perfusion on vascular repair and angiogenesis in osteonecrosis of the femoral head in dogs.
MedLine Citation:
PMID:  23064553     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: The purpose of this study was to observe the effects of marrow mesenchymal stem cell (MSCs) arterial perfusion on vascular repair and angiogenesis in osteonecrosis of the femoral head (ONFH).
METHODS: Twelve healthy male adult Beagle dogs were randomly divided into two groups: group A (the control group) and group B (the MSCs arterial perfusion group). ONFH animal models were established by hip dislocation and liquid nitrogen. At the same time, MSCs were obtained, cultured and proliferated. After three weeks, arterial perfusion was performed in all animals. Group B was given 1 ml MSCs (5 × 10(6)-1 × 10(7)/ml), while 0.9 % normal saline was used in group A. After four weeks or eight weeks, the dogs were put to death. The changes of main arteries, the expression of vascular endothelial growth factor (VEGF), VEGF mRNA and microvessel density (MVD) of ONFH were observed. All the data were analysed by SPSS13.0.
RESULTS: In digital subtraction angiography (DSA), after four or eight weeks of treatment, the quantity and diameter of the main arteries of the femoral head in group B were improved, compared to group A (P < 0.05,P < 0.01). Concerning histology and immunohistochemistry, after four or eight weeks of treatment, the expression of VEGF and MVD were significantly higher than that of group A (P < 0.05, P < 0.01). For real-time quantitative polymerase chain reaction (RT-PCR), after four or eight weeks of treatment, the expression of VEGF mRNA in group B was significantly higher than that of group A (P < 0.05, P < 0.01), and after eight weeks of treatment, the expression of VEGF mRNA were significantly higher than that of four-weeks treatment in group A (P <0.01).
CONCLUSIONS: MSCs arterial perfusion can promote vascular repair and angiogenesis and then improve blood supply and repair of femoral head.
Authors:
Hongting Jin; Bingjiang Xia; Nanze Yu; Bangjian He; Yan Shen; Luwei Xiao; Peijian Tong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-13
Journal Detail:
Title:  International orthopaedics     Volume:  36     ISSN:  1432-5195     ISO Abbreviation:  Int Orthop     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-27     Completed Date:  2013-05-21     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  7705431     Medline TA:  Int Orthop     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2589-96     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Angiography
Animals
Bone Marrow Transplantation / methods*
Disease Models, Animal
Dogs
Femur Head / blood supply*,  metabolism,  physiopathology
Femur Head Necrosis / metabolism,  radiography,  surgery*
Male
Mesenchymal Stem Cell Transplantation / methods*
Microvessels / radiography
Neovascularization, Physiologic / physiology*
Transplantation, Autologous
Treatment Outcome
Vascular Endothelial Growth Factor A / metabolism
Chemical
Reg. No./Substance:
0/Vascular Endothelial Growth Factor A
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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