| The effects of ascorbic acid on dopamine-induced death of PC12 cells are dependent on exposure kinetics. | |
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MedLine Citation:
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PMID: 11108986 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The role of ascorbic acid on dopamine (DA) oxidation-mediated cytotoxicity was studied using the PC12 cell line. DA cytotoxicity was slightly attenuated by ascorbic acid, whereas the cytotoxicity of 6-hydroxydopamine (6-OHDA), a DA oxidation product, was markedly potentiated. To elucidate the relationship between the ascorbic acid effect and the degree of DA oxidation, ascorbic acid was added in a time-dependent fashion after DA treatment. We found greater cell death the later ascorbic acid was applied. Treatment of cells with glutathione alleviated DA- and 6-OHDA-induced cell death, while L-buthionine sulfoximine potentiated DA and 6-OHDA cytotoxicity. Ascorbic acid combined with glutathione eliminated the toxicity of DA and 6-OHDA. These results suggest that the interaction between DA and ascorbic acid is dependent upon the degree of DA oxidation and glutathione availability. |
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Authors:
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H Y Choi; J H Song; D K Park; G M Ross |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neuroscience letters Volume: 296 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 2000 Dec |
Date Detail:
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Created Date: 2000-12-28 Completed Date: 2001-02-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: IRELAND |
Other Details:
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Languages: eng Pagination: 81-4 Citation Subset: IM |
Affiliation:
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Department of Physiology, Queen's University, Kingston, K7L 3N6, Ontario, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Ascorbic Acid / metabolism, pharmacology* Buthionine Sulfoximine / pharmacology Dopamine / metabolism, toxicity* Dose-Response Relationship, Drug Drug Interactions / physiology* Glutathione / metabolism, pharmacology Nerve Degeneration / chemically induced*, metabolism, physiopathology Neuroprotective Agents / metabolism, pharmacology Neurotoxins / metabolism, pharmacology* Oxidative Stress / drug effects*, physiology Oxidopamine / toxicity PC12 Cells / cytology, drug effects*, metabolism Parkinson Disease / etiology, metabolism, physiopathology Pharmacokinetics Rats |
| Chemical | |
Reg. No./Substance:
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0/Neuroprotective Agents; 0/Neurotoxins; 1199-18-4/Oxidopamine; 50-81-7/Ascorbic Acid; 5072-26-4/Buthionine Sulfoximine; 70-18-8/Glutathione |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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