Document Detail

The effects of amiloride, a Na+-H+ exchange inhibitor, on iliac artery stenosis after balloon injury in rabbits.
MedLine Citation:
PMID:  20651838     Owner:  NLM     Status:  MEDLINE    
This study was designed to explore the effects of amiloride, a Na+-H+ exchange (NHE) inhibitor, on vessel stenosis by observing the expression of NHE-1 protein in vascular smooth muscle (VSM) after balloon injury and the effects of amiloride on VSM cell proliferation, migration, and excretion of extracellular matrices (ECMs). A total of 32 adult male New Zealand white rabbits were randomly divided into a balloon injury group (BG), an amiloride-treated group (AG), and a sham-operated group (SG). The left iliac artery was injured by inflating a 2.5 mm x 20 mm Foley catheter in BG and AG rabbits; in SG rabbits, the Foley catheter was inserted but not inflated. Amiloride (5 mg x kg(-1) x d(-1)) was injected intraperitoneally in AG and the same volume of distilled water was used in BG 3 days before balloon injury and for 28 days after the injury. The left iliac artery was stained by hematoxylin-eosin, alpha-actin, and Masson's trichrome to observe the vessel cava, neointima, media layer, and ECMs. NHE-1 proteins of the VSM were detected by Western blotting. A narrowing of the arterial cava, neointima formation, and thickened VSM layer were observed 28 days after balloon injury in BG and AG. However, in AG, the vessel cava was not as narrowed as that of BG and the intimal areas were to a lesser extent than in BG. In AG, the alpha-actin-positive areas and the ECM areas in the neointima were increased compared with SG, but to a lesser extent than in BG. The expression of NHE-1 protein in VSM was increased in BG and AG after balloon injury; however, the levels in AG were significantly less than in BG. In conclusion, VSM cell proliferation, migration, and excretion of ECMs contributed to vessel stenosis in the BG and AG rabbits. The expression of NHE-1 protein in VSM increased after balloon injury. Amiloride, an inhibitor of NHE-1, can limit the development of vessel stenosis through inhibition of VSM cell proliferation, migration, and excretion of ECMs.
Xiao Yi Fang; Ni Yang Lin; Yu Guang Li
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemistry and cell biology = Biochimie et biologie cellulaire     Volume:  88     ISSN:  1208-6002     ISO Abbreviation:  Biochem. Cell Biol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-23     Completed Date:  2010-12-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8606068     Medline TA:  Biochem Cell Biol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  665-70     Citation Subset:  IM    
Department of Pediatrics, First Affiliated Hospital of Shantou University Medical College, Shantou, China.
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MeSH Terms
Amiloride / pharmacology*
Balloon Occlusion / adverse effects*
Cell Movement / drug effects
Constriction, Pathologic
Diuretics / pharmacology
Drug Evaluation, Preclinical
Endothelial Cells / metabolism,  pathology,  physiology
Iliac Artery / drug effects*,  injuries*,  pathology*
Muscle, Smooth, Vascular / drug effects,  metabolism
Peripheral Vascular Diseases / etiology,  metabolism,  pathology
Sodium-Hydrogen Antiporter / antagonists & inhibitors,  metabolism
Tunica Intima / drug effects
Reg. No./Substance:
0/Diuretics; 0/Sodium-Hydrogen Antiporter; 0/growth factor-activatable Na-H exchanger NHE-1; 2609-46-3/Amiloride

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