| The effects of altered levels of phosphatidylcholine and phosphatidylethanolamine on fatty acid desaturase activity and sterol metabolism during temperature acclimation in a choline auxotroph of Neurospora crassa. | |
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MedLine Citation:
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PMID: 6217844 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Experiments were conducted to determine the effects of choline deprivation on levels of phospholipid fatty acids in a choline auxotroph (chol-1; chol-2) of Neurospora crassa with respect to high (37 degrees C) and low (15 degrees C) growth temperatures and during acclimation following a shift from high to low temperature conditions. Although grossly altered levels of phosphatidylethanolamine and phosphatidylcholine were observed at both temperatures, phospholipid fatty acid levels remained virtually identical to those found in a phenotypically wild-type and maximally supplemented chol-1; chol-2 strains grown under the same conditions. Deprivation of choline from supplemented cultures of the mutant followed by a shift from high to low growth temperatures did not significantly affect the level of fatty acid desaturation with respect to control cultures. Free sterols did not significantly affect the level of fatty acid desaturation with respect to control cultures. Free sterols were reduced, however, and sterol ester levels were elevated in choline-deprived cultures, suggesting that sterol interconversions may be closely tied to aspects of phospholipid biosynthesis. These experiments suggest that although major modifications in membrane fluidity may be brought about by thermally induced changes in fatty acid desaturase activity, it seems probable that additional cellular mechanisms may be involved if fluidity is under precise control. |
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Authors:
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A M Johnston; L R Aaronson; C E Martin |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 713 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1982 Dec |
Date Detail:
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Created Date: 1983-03-24 Completed Date: 1983-03-24 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 512-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acclimatization Fatty Acid Desaturases / metabolism* Kinetics Neurospora / metabolism* Neurospora crassa / metabolism* Phosphatidylcholines / metabolism* Phosphatidylethanolamines / metabolism* Sterols / metabolism* Temperature |
| Grant Support | |
ID/Acronym/Agency:
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GM28040/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Sterols; EC 1.14.19.-/Fatty Acid Desaturases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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