Document Detail


The effects of all-trans-retinoic acid on cell cycle and alkaline phosphatase activity in pancreatic cancer cells.
MedLine Citation:
PMID:  17017984     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pancreatic cancer is one of the tumors with the highest mortality, poorly responding to available chemotherapeutic agents. The objective of this study was to study the anticancer effects of all-trans retinoid acid, a functional form of vitamin A, on pancreatic cancer cells. Human pancreatic cancer MiaPaCa-2 cells were treated with 1, 5, 10, 20, 30, 40 and 50 microM ATRA for 1, 2, 3, 4, 5 or 6 d, respectively. Cell growth was determined by MTT viability assay. The cell cycle distribution and the alkaline phosphatase (ALP) activity were analyzed by flow cytometry and chemical analyzer, respectively. The results show that ATRA significantly inhibited the growth of MiaPaCa-2 cells at 40 and 50 microM. ATRA arrested pancreatic cancer cells at G0/G1 phase. The sub-G1 peak and DNA fragmentation were observed. There were time and dose dependent increases in alkaline phosphatase activity (ALP), an indicator of cell differentiation, upon treatment with ATRA when compared to controls. In conclusion, ATRA has an inhibitory effect on the cell growth of MiaPaCa-2, and its tumor suppressive effect is by means of cell cycle arrest and apoptosis induction.
Authors:
J M Guo; B X Xiao; Y R Lou; D H Wang; C H Yan; L Zhan; W H Zhao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medicinal chemistry (Shāriqah (United Arab Emirates))     Volume:  2     ISSN:  1573-4064     ISO Abbreviation:  -     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-10-04     Completed Date:  2007-01-30     Revised Date:  2008-02-26    
Medline Journal Info:
Nlm Unique ID:  101240303     Medline TA:  Med Chem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  457-61     Citation Subset:  IM    
Affiliation:
Ningbo University School of Medicine, Ningbo 315211, China. junmingguo@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / metabolism*
Cell Cycle / drug effects*
Cell Line, Tumor
Flow Cytometry
Humans
Pancreatic Neoplasms / enzymology*,  pathology*
Tretinoin / pharmacology*
Chemical
Reg. No./Substance:
302-79-4/Tretinoin; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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