| The effects of additive solution pH and metabolic rejuvenation on anaerobic storage of red cells. | |
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MedLine Citation:
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PMID: 18631166 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Red cell (RBC) storage can be extended to 9 weeks under anaerobic or alkaline conditions. Simultaneous use of these approaches has not provided additive benefit. Our objective was to determine whether anaerobic storage with acidified additive solution (AS) coupled with metabolic rejuvenation might further improve the benefits of anaerobic storage. STUDY DESIGN AND METHODS: RBC storage in AS with a pH value of 6.5, 7.4, or 8.3 in aerobic or anaerobic conditions was examined using a panel of in vitro biochemical and RBC markers. RBC rejuvenation during cold storage was also evaluated. A randomized crossover radiolabeled recovery study (eight subjects) evaluated anaerobic RBC storage using AS65 with cold rejuvenation for up to 16 weeks of storage. RESULTS: Adenosine triphosphate (ATP) and diphosphoglycerate acid (DPG) were better maintained in anaerobic storage than in aerobic storage. Acidic or neutral AS preserved ATP concentration better, while a neutral or basic pH AS favored maintenance of DPG levels at higher levels for a longer period. AS pH had less of an effect on exposure of phosphatidylserine (PS), vesicle protein release, and hemolysis. Rejuvenation of RBCs during cold, anaerobic storage resulted in increases in ATP and DPG levels and a reversal of PS exposure. Anaerobic storage of RBCs in pH 6.5 AS rejuvenated at 7 weeks of storage yielded RBC 24-hour recoveries of 77.3 +/- 12.5 percent after 10 weeks' storage time. After a second rejuvenation at Week 11, six subjects' units demonstrated a recovery of 75.9 +/- 7.3 percent at 12 weeks of storage. CONCLUSION: Extended RBC storage may be achieved using anaerobic conditions combined with low-pH AS and rejuvenation during storage. |
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Authors:
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Tatsuro Yoshida; James P AuBuchon; Larry J Dumont; James D Gorham; Sean C Gifford; Kevin Y Foster; Mark W Bitensky |
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Publication Detail:
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Type: Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-07-08 |
Journal Detail:
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Title: Transfusion Volume: 48 ISSN: 1537-2995 ISO Abbreviation: Transfusion Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-10-20 Completed Date: 2008-11-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0417360 Medline TA: Transfusion Country: United States |
Other Details:
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Languages: eng Pagination: 2096-105 Citation Subset: IM |
Affiliation:
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Biomedical Engineering Department, Boston University College of Engineering, Boston, Massachusetts 02215, USA. tatsuro.yoshida@newhealthsciences.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Aerobiosis Alkalies Anaerobiosis* Blood Preservation / methods* Blood Transfusion Cell Survival Cross-Over Studies Cryopreservation / methods* Diphosphoglyceric Acids / metabolism Erythrocytes / cytology*, metabolism Hemolysis Humans Hydrogen-Ion Concentration Organ Preservation Solutions / pharmacology* Phosphatidylserines / metabolism Pilot Projects Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Alkalies; 0/Diphosphoglyceric Acids; 0/Organ Preservation Solutions; 0/Phosphatidylserines; 56-65-5/Adenosine Triphosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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