Document Detail


The effects of accumulation of an environmentally relevant polychlorinated biphenyl mixture on cytochrome P450 and P-glycoprotein expressions in fetuses and pregnant rats.
MedLine Citation:
PMID:  19264347     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to improve knowledge about transplacental transfer of an environmentally relevant PCB mixture by evaluating congener levels in livers and brains of rat dams and fetuses after maternal exposure, and correlating them to the levels of CYP450 and P-glycoprotein, involved in biotransformation and xenobiotics export, respectively. Pregnant dams were injected daily from gestation day (GD) 15 to 19 with 10mgkg(-1) of a reconstituted mixture (RM) composed of PCB138, 153, 180 and 126. Our data indicate that at GD20 RM is partitioned among maternal tissues, and that fetuses are not excluded from this distribution, evidencing a placental transfer of PCBs. Considering the ratio of maternal and fetal PCB concentrations based on lipid-weight, the amounts of congeners were 7-fold lower in fetal livers than in maternal livers and 25-30-fold higher in fetal brains than in maternal ones. Moreover, in dams the congeners were able to induce hepatic CYP450 response (total CYP450, CYP1A and CYP2B), but failed to increase P-170 expression, while in fetuses the constitutive expression of CYP450 and P-170 was not induced by treatment. Pearson Product-Moment Correlation applied to treated group data suggests that PCB accumulation in fetal livers, but not in brains, depended principally on their mothers' intoxication pattern. On the whole, these results emphasize the maternal liver and the fetal brain as depot organs for PCB sequestration and their susceptibility towards PCB toxicological risk. Moreover they highlight the lack of a coordinated response between the investigated defence mechanism.
Authors:
Patrizia Bonfanti; Anita Colombo; Sara Villa; Francesca Comelli; Barbara Costa; Angela Santagostino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-04
Journal Detail:
Title:  Chemosphere     Volume:  75     ISSN:  1879-1298     ISO Abbreviation:  Chemosphere     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-13     Completed Date:  2009-06-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0320657     Medline TA:  Chemosphere     Country:  England    
Other Details:
Languages:  eng     Pagination:  572-9     Citation Subset:  IM    
Affiliation:
Department of Environmental Sciences, University of Milano-Bicocca, Italy. patrizia.bonfanti@unimib.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Biotransformation
Brain / metabolism
Brain Chemistry
Cytochrome P-450 Enzyme System / metabolism*
Environmental Pollutants / metabolism*
Female
Fetus / metabolism*
Liver / chemistry,  metabolism
P-Glycoprotein / metabolism*
Polychlorinated Biphenyls / analysis*
Pregnancy
Rats
Rats, Sprague-Dawley
Xenobiotics / analysis
Chemical
Reg. No./Substance:
0/Environmental Pollutants; 0/P-Glycoprotein; 0/Polychlorinated Biphenyls; 0/Xenobiotics; 9035-51-2/Cytochrome P-450 Enzyme System

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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