Document Detail


The effects of GATA-1 and NF-E2 deficiency on bone biomechanical, biochemical, and mineral properties.
MedLine Citation:
PMID:  23359245     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mice deficient in GATA-1 or NF-E2, transcription factors required for normal megakaryocyte (MK) development, have increased numbers of MKs, reduced numbers of platelets, and a striking high bone mass phenotype. Here, we show the bone geometry, microarchitecture, biomechanical, biochemical, and mineral properties from these mutant mice. We found that the outer geometry of the mutant bones was similar to controls, but that both mutants had a striking increase in total bone area (up to a 35% increase) and trabecular bone area (up to a 19% increase). Interestingly, only the NF-E2 deficient mice had a significant increase in cortical bone area (21%) and cortical thickness (27%), which is consistent with the increase in bone mineral density (BMD) seen only in the NF-E2 deficient femurs. Both mutant femurs exhibited significant increases in several biomechanical properties including peak load (up to a 32% increase) and stiffness (up to a 13% increase). Importantly, the data also demonstrate differences between the two mutant mice. GATA-1 deficient femurs break in a ductile manner, whereas NF-E2 deficient femurs are brittle in nature. To better understand these differences, we examined the mineral properties of these bones. Although none of the parameters measured were different between the NF-E2 deficient and control mice, an increase in calcium (21%) and an increase in the mineral/matrix ratio (32%) was observed in GATA-1 deficient mice. These findings appear to contradict biomechanical findings, suggesting the need for further research into the mechanisms by which GATA-1 and NF-E2 deficiency alter the material properties of bone.
Authors:
Melissa A Kacena; Caren M Gundberg; William J Kacena; William J Landis; Adele L Boskey; Mary L Bouxsein; Mark C Horowitz
Related Documents :
23145915 - Fragmented adipose tissue transplanted to craniofacial deformities induces bone repair ...
23706035 - Mechanical evaluation of a tissue-engineered zone of calcification in a bone-hydrogel o...
23336755 - One-stage reconstruction of full-thickness lower eyelid using a tripier flap lining by ...
23303515 - Split and thinned pedicle deep inferior epigastric perforator (diep) flap for vulvar re...
6778065 - Ectopic bone formation following charnley hip arthroplasty.
18442055 - Comparison of sensory recovery and morphologic change between sensate and nonsensate fl...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  228     ISSN:  1097-4652     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-08-05     Completed Date:  2013-11-13     Revised Date:  2013-12-16    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1594-600     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Biomechanical Phenomena
Bone Density / physiology*
Bone and Bones / anatomy & histology,  physiology*
Calcium / metabolism
Female
Femur / anatomy & histology,  physiology
GATA1 Transcription Factor / deficiency*,  genetics,  metabolism
Megakaryocytes / cytology,  metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
NF-E2 Transcription Factor, p45 Subunit / deficiency*,  genetics,  metabolism
Osteoblasts / cytology,  metabolism
Grant Support
ID/Acronym/Agency:
P30 AR041621/AR/NIAMS NIH HHS; P30 AR046032/AR/NIAMS NIH HHS; P30 AR046121/AR/NIAMS NIH HHS; P30 AR46032/AR/NIAMS NIH HHS; R01 AR060332/AR/NIAMS NIH HHS; R01 AR060332/AR/NIAMS NIH HHS; R01 AR38460/AR/NIAMS NIH HHS; R01 AR47342/AR/NIAMS NIH HHS; R03 AR055269/AR/NIAMS NIH HHS; R03 AR055269/AR/NIAMS NIH HHS; S10 RR017868/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/GATA1 Transcription Factor; 0/Gata1 protein, mouse; 0/NF-E2 Transcription Factor, p45 Subunit; 0/Nfe2 protein, mouse; SY7Q814VUP/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Impact of subject head motion on quantitative brain (15)O PET and its correction by image-based regi...
Next Document:  Children's Enjoyment of Play During School Lunchtime Breaks: An Examination of Intra- and Inter-Day ...