Document Detail


The effects of 5-bromodeoxyuridine on the growth and morphology of transformed rat liver cells.
MedLine Citation:
PMID:  7440648     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of bromodeoxyuridine (BrdUrd) on the growth, morphology, and tumorigenicity of the spontaneously transformed rat liver cell line R72/3 were studied. These cells grow either in suspension or in a monolayer and are tumorigenic. In monolayer cultures, cells treated with low concentrations (2.5 micrograms/ml) of BrdUrd were larger, more spread out, and more firmly attached to the substratum than were untreated controls. Treated cells failed to grow in suspension or on confluent monolayers of 3T3 cells and did not form colonies in soft agar. Scanning electron microscopy revealed extensive flattening of treated cells and a dramatic reduction in the number of microvilli on the cell surface. Transmission electron microscopy showed an increase in polyribosomes and rough endoplasmic reticulum, as well as an enlargement of endoplasmic reticulum cisternae and a complete absence of the bundles of intermediate size filaments that were conspicuous in untreated cells. The persistence of these changes required the continuous presence of BrdUrd in the medium. The effects of BrdUrd were readily reversed by withdrawal of BrdUrd and were not expressed in the presence of excess thymidine.
Authors:
N Avdalovic; J Weibel; L Diamond
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  104     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1980 Jul 
Date Detail:
Created Date:  1981-02-24     Completed Date:  1981-02-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  83-96     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bromodeoxyuridine / antagonists & inhibitors,  pharmacology*
Cell Differentiation
Cell Line
Dose-Response Relationship, Drug
Epithelium / ultrastructure*
Liver
Microscopy, Electron
Microscopy, Electron, Scanning
Rats
Thymidine / pharmacology
Grant Support
ID/Acronym/Agency:
CA-08936/CA/NCI NIH HHS; CA-10815/CA/NCI NIH HHS; CA-21778/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
50-89-5/Thymidine; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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