Document Detail


The effects of 17beta-estradiol and vitamin E treatments on oxidative stress and antioxidant levels in brain cortex of diabetic ovariectomized rats.
MedLine Citation:
PMID:  20511130     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the effects of 17beta-estradiol (E 2 ) and vitamin E on lipid peroxidation and antioxidant levels in brain cortex of diabetic ovariectomized (OVX) rats. Forty-two rats were equally divided into seven groups: Control; OVX; OVX+E 2 ; OVX+E 2 +vitamin E; OVX+diabetic; OVX+diabetic+E 2 and OVX+diabetic+E 2 +vitamin E. Then, E 2 (40 microg kg -1 /day) and vitamin E (100 mg kg -1 /day) were given. Glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), vitamin A and beta-carotene in brain were decreased (p<0.05) when compared with control in OVX but MDA in brain and glucose in plasma were elevated (p<0.05). The antioxidant levels in brain (p<0.05) increased although MDA (p<0.05) in brain and glucose (p<0.05, p<0.01) in plasma decreased in OVX after E 2 and E 2 +vitamin E supplementation. The activities of GSH-Px (p<0.001, p<0.01), CAT (p<005, p<0.001) and SOD (p<0.001) and the levels of GSH (p<0.001), vitamin A (p<0.05) and beta-carotene (p<0.001, p<0.05) were lower in the brain of OVX diabetic rats, while MDA in the brain and glucose in the plasma were higher (p<0.001). The antioxidant enzymes (p<0.05, p<0.01), GSH, vitamin A and beta-carotene (p<0.01, p<0.001) in brain increased while MDA in brain and glucose in plasma decreased (p<0.01, p<0.001) in diabetic OVX after treatments. Finally, the E 2 and E 2 +vitamin E supplementation to diabetic OVX and OVX rats may strengthen the antioxidant system by reducing lipid peroxidation.
Authors:
M Ulas; M Cay
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta physiologica Hungarica     Volume:  97     ISSN:  0231-424X     ISO Abbreviation:  Acta Physiol Hung     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-31     Completed Date:  2010-07-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309201     Medline TA:  Acta Physiol Hung     Country:  Hungary    
Other Details:
Languages:  eng     Pagination:  208-15     Citation Subset:  IM    
Affiliation:
Department of Physiology, Faculty of Medicine, Firat University, Elazig, Turkey. ulas.mustafa@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / administration & dosage*,  metabolism
Blood Glucose / metabolism
Catalase / metabolism
Cerebral Cortex / drug effects*,  metabolism
Diabetes Mellitus, Experimental / drug therapy*,  metabolism
Estradiol / administration & dosage*
Female
Glutathione / metabolism
Glutathione Peroxidase / metabolism
Lipid Peroxidation / drug effects
Malondialdehyde / metabolism
Ovariectomy*
Oxidative Stress / drug effects*
Rats
Rats, Wistar
Superoxide Dismutase / metabolism
Vitamin A / metabolism
Vitamin E / administration & dosage*
beta Carotene / metabolism
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Blood Glucose; 11103-57-4/Vitamin A; 1406-18-4/Vitamin E; 50-28-2/Estradiol; 542-78-9/Malondialdehyde; 70-18-8/Glutathione; 7235-40-7/beta Carotene; EC 1.11.1.6/Catalase; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase

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