Document Detail


The effect of zidovudine and 2'3'-dideoxyinosine on human trophoblast in culture.
MedLine Citation:
PMID:  7753766     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Trophoblast from term and first trimester placenta, maintained in culture, were exposed to 20 mumoles/l zidovudine or 2'3'dideoxyinosine. Several indices of function were measured and compared to control trophoblast in parallel culture. The results from individual placentas were examined by Student's t-test and cumulative results by ANOVA. Neither zidovudine or 2'3'-dideoxyinosine had statistically significant effects on the function of term trophoblast, following a 48 hr exposure to the drug as indicated by hCG secretion, protein synthesis and glucose consumption. In one of five placentas exposed to zidovudine, progesterone secretion was reduced as compared to its control but remained in the high range. Zidovudine had no significant effect on cultured trophoblast isolated from first trimester placenta even after prolonged exposure to the drug for eleven days. Both term and first trimester trophoblast in culture tolerate prolonged exposure to high concentrations of zidovudine or 2'3'-dideoxyinosine. Human trophoblast in culture provides a safe in vitro model for the screening of drugs intended for use during pregnancy.
Authors:
A L Esterman; C Rosenberg; T Brown; J Dancis
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Pharmacology & toxicology     Volume:  76     ISSN:  0901-9928     ISO Abbreviation:  Pharmacol. Toxicol.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-06-22     Completed Date:  1995-06-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8702180     Medline TA:  Pharmacol Toxicol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  89-92     Citation Subset:  IM    
Affiliation:
Department of Paediatrics, New York University School of Medicine, U.S.A.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Cells, Cultured
Chorionic Gonadotropin / secretion
Didanosine / pharmacology*
Energy Metabolism / drug effects
Female
Humans
Pregnancy
Pregnancy Proteins / biosynthesis,  drug effects,  secretion
Pregnancy Trimester, First
Pregnancy Trimester, Third
Progesterone / secretion
Trophoblasts / drug effects*,  secretion
Zidovudine / toxicity*
Grant Support
ID/Acronym/Agency:
A1-29349//PHS HHS; A1-32298//PHS HHS
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Pregnancy Proteins; 30516-87-1/Zidovudine; 57-83-0/Progesterone; 69655-05-6/Didanosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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