| The effect of weight loss on protein profiles of gastrocnemius muscle in rabbits: a study using 1D electrophoresis and peptide mass fingerprinting. | |
| | |
MedLine Citation:
|
PMID: 19175456 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The study of physiological changes occurring during selection contributes to an improved understanding of relationships leading to efficiencies in animal production. To investigate the effects of food restriction in gastrocnemius muscle protein expression, 20% weight reduction was induced in New Zealand White (meat producing) and wild rabbits, using one-dimensional gel electrophoresis and peptide mass fingerprinting. Lower expression levels of myosin heavy chains were found in the Wild Rabbits Restricted Group, while myosin light chain and alpha-crystallin proteins were not detected in restricted groups. Glyceraldeyde-3-phosphate dehydrogenase and glycogen phosphorylase expression levels were similar for all experimental groups. Phosphopyruvate hydratase beta was not detected in the wild rabbit restricted diet group. Pyruvate kinase levels were 50% lower in the New Zealand Restricted group. LIM protein detection was absent in the control New Zealand group. Results also show relevance of actin in preserving muscle structure in depressed food availability, the sensitivity of both myosin light chain and alpha-crystallin protein to restricted feed and the role of PK in the resistance of New Zealand rabbits to food restriction. |
| | |
Authors:
|
A M Almeida; S van Harten; A Campos; A V Coelho; L A Cardoso |
Related Documents
:
|
7861246 - Activities of antioxidant enzymes in various tissues of male fischer 344 rats are alter... 1812086 - Effect of riboflavin or pyridoxine deficiency on inflammatory response. 8914486 - The evolution of the antiaging action of dietary restriction: a hypothesis. 17490786 - Do not eat the red food!: prohibition of snacks leads to their relatively higher consum... 16770766 - The ontogeny of postingestive inhibitory stimuli: examining the role of cck. 8084646 - Environmental control of allergens. |
Publication Detail:
|
Type: Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't Date: 2009-01-13 |
Journal Detail:
|
Title: Journal of animal physiology and animal nutrition Volume: 94 ISSN: 1439-0396 ISO Abbreviation: J Anim Physiol Anim Nutr (Berl) Publication Date: 2010 Apr |
Date Detail:
|
Created Date: 2010-05-14 Completed Date: 2010-08-30 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101126979 Medline TA: J Anim Physiol Anim Nutr (Berl) Country: Germany |
Other Details:
|
Languages: eng Pagination: 174-85 Citation Subset: IM |
Affiliation:
|
Instituto de Investigação Científica Tropical & Centro Interdisciplinar de Investigação em Sanidade Animal, Lisboa, Portugal. aalmeida@fmv.utl.pt |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blood Glucose Electrophoresis / methods, veterinary* Food Deprivation Gene Expression Regulation / genetics, physiology Insulin / blood Muscle Proteins / genetics, metabolism* Muscle, Skeletal / metabolism* Peptide Mapping / methods, veterinary* Rabbits / genetics*, metabolism Weight Loss / physiology* |
| Chemical | |
Reg. No./Substance:
|
0/Blood Glucose; 0/Muscle Proteins; 11061-68-0/Insulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Long-term feeding effects of dietary protein levels on egg production, immunocompetence and plasma a...
Next Document: Effect of induced body condition score differences on physiological response, productive and reprodu...