| The effect of ursodeoxycholic acid on the survivin in thapsigargin-induced apoptosis. | |
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MedLine Citation:
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PMID: 12609713 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Endoplasmic reticulum (ER) was recently suggested as a third subcellular compartment in apoptotic execution. Survivin is a member of inhibitors of apoptosis and ursodeoxycholic acid (UDCA) prevents apoptosis from various apoptotic stimuli. To assess the activity of survivin and the effect of UDCA on the survivin in ER stress-mediated apoptosis, we treated hepatoma cell lines with thapsigargin (TG). TG-induced apoptosis was assessed by morphological changes, DNA fragmentation, cleavages of poly(ADP-ribose)polymerase (PARP), and activation of calpain and caspase-12. The level of survivin was decreased after TG treatment in hepatoma cell lines indicating that survivin play an important role in ER stress-mediated apoptosis. UDCA prevented decrease in survivin levels and inhibited TG-induced apoptosis and caspase-12 activation suggesting an anti-apoptotic effect of UDCA. |
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Authors:
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Joohyun Sohn; Vladimir I Khaoustov; Qing Xie; Charles C Chung; Bhuvaneswari Krishnan; Boris Yoffe |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Cancer letters Volume: 191 ISSN: 0304-3835 ISO Abbreviation: Cancer Lett. Publication Date: 2003 Feb |
Date Detail:
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Created Date: 2003-02-28 Completed Date: 2003-05-08 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 83-92 Citation Subset: IM |
Affiliation:
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Department of Medicine, Baylor College of Medicine and Veterans Affairs Medical Center (151B), 2002 Holcombe Boulevard, Houston, TX 77030, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects*,
physiology Calpain / metabolism Carcinoma, Hepatocellular / pathology Caspase 12 Caspases / metabolism DNA Fragmentation / drug effects Endoplasmic Reticulum / physiology Enzyme Activation / drug effects Genes, bcl-2 Humans Liver Neoplasms / pathology Microtubule-Associated Proteins / biosynthesis*, genetics, physiology Neoplasm Proteins / biosynthesis*, genetics, physiology Poly(ADP-ribose) Polymerases / metabolism Proto-Oncogene Proteins c-bcl-2 / deficiency, physiology Stress, Physiological / genetics, metabolism Thapsigargin / pharmacology* Tumor Cells, Cultured / drug effects, metabolism, pathology Ursodeoxycholic Acid / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/BIRC5 protein, human; 0/CASP12 protein, human; 0/Microtubule-Associated Proteins; 0/Neoplasm Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 128-13-2/Ursodeoxycholic Acid; 67526-95-8/Thapsigargin; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Calpain; EC 3.4.22.-/Caspase 12; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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