Document Detail


The effect of ultrasonic irradiation on doxorubicin-induced cytotoxicity in three human bladder cancer cell lines.
MedLine Citation:
PMID:  17173946     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although ultrasonic irradiation has been proven to increase membrane permeability and enhance chemotherapeutic cytotoxicity in a number of cell lines, this effect has never been demonstrated in bladder cancer cells. Bladder cancer may offer a unique setting for ultrasound enhancement of chemotherapy, since intravesicular rather than intravenous administration of chemotherapy is used in superficial cases. The aim of this study was to investigate whether a non-toxic dose of ultrasound could increase membrane permeability, and potentiate the cytotoxicity of doxorubicin to three human bladder carcinoma cell lines (TCC-SUP, T24, and RT4) in vitro. An EuTDA-Efflux assay, which measures the amount of a chemical that is allowed to seep out of labeled cells, was used to analyze membrane permeability, and an MTS assay, which directly measures cell viability, was used to determine the effect of chemotherapy on cells after they were treated with a variety of doxorubicin concentrations and ultrasonic exposures. Ultrasound treatment for 5min and 10min at an intensity of approximately 0.3W/cm(2) resulted in a significant increase in EuTDA efflux in all three cell lines. However, no ultrasonic enhancement of doxorubicin growth inhibition in these human bladder carcinoma cells was observed. This suggests that either ultrasound does not increase doxorubicin uptake by the cell or that doxorubicin uptake is increased but in insufficient amounts to affect growth inhibition. Further investigation should focus on explaining these results.
Authors:
Caroline Arthur; Thomas Flaig; Lih-Jen Su; Richard Denney; Frank Barnes; L Michael Glodé
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2006-11-27
Journal Detail:
Title:  Ultrasonics     Volume:  46     ISSN:  1874-9968     ISO Abbreviation:  Ultrasonics     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-13     Completed Date:  2007-03-07     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0050452     Medline TA:  Ultrasonics     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  68-73     Citation Subset:  IM    
Affiliation:
Washington University School of Medicine, St. Louis, MO, USA.
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MeSH Terms
Descriptor/Qualifier:
Antibiotics, Antineoplastic / therapeutic use*
Cell Line, Tumor
Cell Membrane Permeability
Cell Survival / drug effects
Combined Modality Therapy / methods
Dose-Response Relationship, Drug
Doxorubicin / therapeutic use*
Humans
Treatment Outcome
Urinary Bladder Neoplasms / drug therapy*,  pathology,  ultrasonography*
Grant Support
ID/Acronym/Agency:
R25 CA049981/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 23214-92-8/Doxorubicin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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