Document Detail


The effect of tuberculin skin test and BCG vaccination on the expansion of PPD-specific IFN-gamma producing cells ex vivo.
MedLine Citation:
PMID:  18023944     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Understanding the immunogenicity of BCG in a population where it has failed will facilitate the design of new TB vaccines. We assessed the immunogenicity of M. bovis BCG over 12 months by ELISPOT assay. Forty-one adolescents and young Gambian male adults received a tuberculin skin test (TST) which was followed one week later by BCG vaccination, but the 23 control subjects received neither of these. TST alone significantly induced PPD-specific IFN-gamma producing cells. Twenty-three percent of subjects did not respond to BCG, which was associated with higher pre-existing ex vivo response to PPD. Paradoxically, amongst BCG responders there was a correlation between pre-existing response and subsequent response to BCG. We conclude that BCG is immunogenic, but this effector response is short-lived and can be limited in higher pre-existing anti-mycobacterial immunity, suggesting a possible threshold beyond which BCG immunogenicity is inhibited.
Authors:
Martin O C Ota; Roger H Brookes; Philip C Hill; Patrick K Owiafe; Hannah B Ibanga; Simon Donkor; Timothy Awine; Helen McShane; Richard A Adegbola
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-31
Journal Detail:
Title:  Vaccine     Volume:  25     ISSN:  0264-410X     ISO Abbreviation:  Vaccine     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-10     Completed Date:  2008-02-21     Revised Date:  2010-05-07    
Medline Journal Info:
Nlm Unique ID:  8406899     Medline TA:  Vaccine     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  8861-7     Citation Subset:  IM    
Affiliation:
Bacterial Diseases Programme, Medical Research Council, P.O. Box 273, Banjul, Gambia. mota@mrc.gm
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Cells, Cultured
Gambia
Humans
Interferon-gamma / biosynthesis*
Leukocytes, Mononuclear / immunology
Lymphocytes / immunology*
Male
Mycobacterium bovis / immunology*
Tuberculin Test*
Tuberculosis Vaccines / immunology*
Grant Support
ID/Acronym/Agency:
076943//Wellcome Trust
Chemical
Reg. No./Substance:
0/Tuberculosis Vaccines; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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