| The effect of temperature during trypsin treatment on viability and multiplication potential of single normal human and chicken fibroblasts. | |
| | |
MedLine Citation:
|
PMID: 610874 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Both the number of colonies formed from single cells and their average size are affected by the temperature at which nontransformed human and chicken fibroblasts are harvested from monolayer cultures. At constant levels of trypsin activity, treatment of cells at temperatures less than 15 degrees C during all steps from monolayer to subsequent culture markedly improves viability and multiplication potential of single cells. |
| | |
Authors:
|
W L McKeehan |
Related Documents
:
|
11902774 - Factors affecting seasonal variation of membrane filtration resistance caused by chlore... 57944 - Properties and kinetics of development of rous sarcoma virus-infected cells evidenced b... 17603774 - Culture and spray-drying of tsukamurella paurometabola c-924: stability of formulated p... 12656854 - The effect of age of refrigerated conditioned medium on cell survivability in vitro. 23437164 - Using phenotype microarrays to determine culture conditions that induce or repress toxi... 19563254 - Spontaneous formation of highly functional three-dimensional multilayer from human hepa... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Cell biology international reports Volume: 1 ISSN: 0309-1651 ISO Abbreviation: Cell Biol. Int. Rep. Publication Date: 1977 Jul |
Date Detail:
|
Created Date: 1978-06-12 Completed Date: 1978-06-12 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7708050 Medline TA: Cell Biol Int Rep Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 335-43 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Adhesion Cell Division / drug effects* Cell Survival / drug effects* Cells, Cultured Clone Cells Temperature Trypsin / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: DNA distribution and intrachromosomal organization of moderately repetitive sequences.
Next Document: Cytoplasmic annulate lamellae and intranuclear membranes in human spermatids and sperm.