Document Detail


The effect of temperature during trypsin treatment on viability and multiplication potential of single normal human and chicken fibroblasts.
MedLine Citation:
PMID:  610874     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Both the number of colonies formed from single cells and their average size are affected by the temperature at which nontransformed human and chicken fibroblasts are harvested from monolayer cultures. At constant levels of trypsin activity, treatment of cells at temperatures less than 15 degrees C during all steps from monolayer to subsequent culture markedly improves viability and multiplication potential of single cells.
Authors:
W L McKeehan
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell biology international reports     Volume:  1     ISSN:  0309-1651     ISO Abbreviation:  Cell Biol. Int. Rep.     Publication Date:  1977 Jul 
Date Detail:
Created Date:  1978-06-12     Completed Date:  1978-06-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7708050     Medline TA:  Cell Biol Int Rep     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  335-43     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Adhesion
Cell Division / drug effects*
Cell Survival / drug effects*
Cells, Cultured
Clone Cells
Temperature
Trypsin / pharmacology*
Chemical
Reg. No./Substance:
EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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